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Hung-Chi J. Chen, Lung-Kun Yeh, Chi-Chin Sun, Scheffer C. Tseng, Jan-Kan Chen, David H. Ma; Expression of Angiogenesis-related Factors in Human Corneas after Cultivated Oral Mucosal Epithelial Transplantation. Invest. Ophthalmol. Vis. Sci. 2012;53(14):269.
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© ARVO (1962-2015); The Authors (2016-present)
To analyze the expression of angiogenesis-related factors in corneal tissues previously undergoing autologous cultivated oral mucosal epithelial transplantation (COMET).
Six eyes from four chemically and two thermally injured patients with limbal stem cell deficiency who received COMET to promote wound healing were retrospectively studied. Immunoconfocal microscopy was performed on corneal specimens from above-mentioned patients after COMET as well as normal cornea and oral mucosa for keratin 8 (K-8), fibroblast growth factor-2 (FGF-2), vascular endothelial growth factor (VEGF), collagen XVIII (endostatin), pigment epithelium-derived factor (PEDF), soluble fms-like tyrosine kinase-1 (sFlt-1), tissue inhibitor of metalloprotease-3 (TIMP-3), thrombospondin-1 (TSP-1), and interleukin-1 receptor antagonist (IL-1ra).
FGF-2, VEGF, endostatin, PEDF, and IL-1ra were universally detected in all samples, among which signal for FGF-2, VEGF and IL-1ra was localized to full-thickness epithelial layer, while endostatin was limited to the basement membrane. Expression of PEDF varied in tissues from all six patients, with a preferential expression in the suprabasal epithelial layer. FGF-2 and IL-1ra were over-expressed in basal epithelial layer in specimens with increased stratification. Signal for sFlt-1, TIMP-3, and TSP-1 was positive only in normal cornea and corneal epithelium in the specimen from Patient 3, while it was negative in all other specimens.
Immunoconfocal expression of FGF-2, VEGF, PEDF, endostatin, and IL-1ra was similar in normal cornea, oral mucosa, and corneas after COMET. Expression of sFlt-1, TIMP-3, and TSP-1 was negligible in oral mucosal epithelial cells and corneas after COMET. Lack of above-mentioned anti-angiogenic factors may contribute to peripheral corneal neovascularization often seen after COMET.
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