Purchase this article with an account.
Tao L. Lowe, Linfeng Wu, Keegan B. Compton, Junjie Zhang; Development of Hyaluronic Acid Hydrogels for Ocular Stem Cell Differentiation. Invest. Ophthalmol. Vis. Sci. 2012;53(14):317.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The purpose of this study is to design hyaluronic acid (HA) based hydrogels to differentiate ocular stem cells for ocular tissue regeneration.
A serial of hyaluronic acid macromers are synthesized by conjugating 2-aminoethyl methacrylate (AEMA) to hyaluronic acid with different molecular weights (i.e. 6400, 16000 and 66000). The chemical structures of these macromers are characterized using ATR-FTIR and NMR. Their cytotoxicity is tested to different cell lines such as PC-12 and ocular stem cells using the MTT assay. Hyaluronic acid hydrogels are synthesized from these macromers using photo-polymerization. The mechanical properties of these hydrogels are characterized using rheometer and dynamic mechanical analyzer (DMA). The degradation of these hydrogels is also characterized. PC-12 cells and ocular stem cells such as limbal stem cells are encapsulated into the hydrogels in situ during the photo-polymerization. The cell encapsulated hydrogels are stained using LIVE/DEAD® cell viability assays to determine the cell viability after photo-polymerization. In particular, the differential of the ocular stem cells are induced using several different growth factors. The efficiency of induced differentiation is under investigation.
ATR-FTIR and NMR measurements confirm the successful synthesis of HA-AEMA macromers. The substitution degree can be modulated by adjusting the molecular weight of HA and the ratio of AEMA to HA. MTT data indicate that these HA-AEMA macromers are not cytotoxic. Hydrogels can be formed under mild photo-polymerization conditions, while LIVE/DEAD cell stain indicates that cells could survive under such polymerization conditions. The growth factor induced differentiation of ocular stem cells is right now under investigation.
The recently developed HA hydrogels have great potential for in situ encapsulation of ocular stem cells and differentiation of these cells for ocular tissue regeneration.
This PDF is available to Subscribers Only