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Vijay Khetpal, S Balaiya, K V. Chalam; Resveratrol inhibits Choroidal Endothelial Cell Proliferation through the induction of SAPK/JNK Pathway. Invest. Ophthalmol. Vis. Sci. 2012;53(14):442.
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Exudative AMD results from hypoxia induced choroidal neovascularization. Resveratrol, a common antioxidant present in red wine and natural plants is anti-angiogenic in carcinoma and pro-angiogenic in experimental models of vascular endothelial cells. It decelerate the aging process and modulate vascular endothelial cell function in diverse angiogenic beds. In this study, we investigated the effects of resveratrol on cell viability and its effects on apoptosis in hypoxic choroidal endothelial cells.
Choroidal endothelial cells (RF/6A) where exposed to escalating doses of resveratrol 4 and 12 µg/ml and cell viability was analysed by WST-1 assay. Hypoxia was induced through cobalt chloride (200 µM) and the effect of resveratrol (4 and 12µg/ml) was evaluated by assessing Stress Activated ProteinKinase/c-Jun N-terminal Kinase (SAPK/JNK) pathway using immunoblot and densitometric analysis.
In the presence of hypoxia (200µM of cobalt chloride), cell proliferation increased to 129.3±1.13% whereas it decreased to 125.6±1.02% after the addition of resveratrol at 4µg/ml and 86.1±2.16% at 12µg/ml of concentration. In comparison to basal level (0.3% ODU), phosphorylation state of SAPK/JNK increased after hypoxic insult to 5.3% at 200 µM concentration of cobalt chloride which decreased to 3.8 and 2.5% after the addition of 4 and 12 µg/mL of resveratrol, respectively. (P<0.01)
Resveratrol inhibits proliferation of hypoxic choroidal endothelial cells and its apoptotic effects may be relayed through the SAPK/JNK pathway.
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