March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Ultrasound-enhanced Penetration Of Topical Fluorescein into the Vitreous in Rabbit Eyes
Author Affiliations & Notes
  • Jay M. Stewart
    Ophthalmology,
    Univ of California-San Francisco, San Francisco, California
  • Chris J. Diederich
    Radiation Oncology,
    Univ of California-San Francisco, San Francisco, California
  • Elliot Chan
    Ophthalmology,
    Univ of California-San Francisco, San Francisco, California
  • Vasant Salgaonkar
    Radiation Oncology,
    Univ of California-San Francisco, San Francisco, California
  • Ricardo Lamy
    Ophthalmology,
    Univ of California-San Francisco, San Francisco, California
  • Footnotes
    Commercial Relationships  Jay M. Stewart, None; Chris J. Diederich, None; Elliot Chan, None; Vasant Salgaonkar, None; Ricardo Lamy, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 493. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jay M. Stewart, Chris J. Diederich, Elliot Chan, Vasant Salgaonkar, Ricardo Lamy; Ultrasound-enhanced Penetration Of Topical Fluorescein into the Vitreous in Rabbit Eyes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):493.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine whether ultrasound treatment can promote the permeation of topical fluorescein into the vitreous.

Methods: : Fresh cadaveric rabbit eyes were placed in saline solution at 37C. An eye cup containing 0.1% fluorescein solution was centered on the sclera approximately 5 mm posterior to the limbus. Continuous wave ultrasound at 1 W/cm2 was applied to the sclera within the eye cup for 10 minutes, and the eyes were then left in solution for an additional 50 minutes. Control eyes received the same exposure to fluorescein solution in the eye cup without ultrasound treatment. Eyes were then washed, and vitreous was collected using an automated vitrectomy system. Vitreous samples were analyzed using fluorescence spectophotometry. Fluorescence intensity levels were compared to a standard curve representing known fluorescein concentrations.

Results: : The vitreous concentration of fluorescein was increased in treated eyes (mean 4.34 mcg/mL, n=10) compared to control eyes (mean 2.88 mcg/mL, n=8), P < 0.05.

Conclusions: : Ultrasound treatment facilitated the entry of topical fluorescein into the vitreous of intact rabbit eyes. These results suggest that ultrasound-enhanced drug delivery may offer a non-invasive means of treating posterior segment diseases.

Keywords: retina • vitreous • drug toxicity/drug effects 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×