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Wei Li, Yihui Mao, Yangluowa Qu, Jing Jie, Zhixing Geng, Hua Wang, Wensheng Chen, Hui He, Jie Yang, Zuguo Liu; Pathological Changes Of Hair Follicle And Meibomian Gland In Demodex Related Blepharitis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):610.
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Demodex inhabits in hair follicle, sebaceous gland and meibomian gland. More and more studies revealed high relevance of demodex in ocular surface diseases such as chronic blepharitis, onjunctivitis, and meibomian gland dysfunction. However, the exact pathogenic mechanism remains elusive. This study is subjected to investigate the pathogenic role of demodex on hair follicle and meibomian gland in blepharitis.
Eyelashes from 20 patients of demodex blepharitis and 15 healthy volunteers, and eyelid tissue from 6 cadavers were studied. Apoptosis of hair follicle was determined by TUNEL assay. Ki67 and TUNEL double staining were performed on eyelid cross-sections. Epithelial differentiation of hair follicle and meibomian gland was determined by immunostaining of K10, K14, K15, and K16. Inflammatory cell infiltration was determined by CD11c and MAC387 immunostaining on eyelid tissues.
The apoptotic cells increased in hair follicle and surrounding tissue in demodex blepharitis. K10 expression extended into deep hair follicle, glandular tube and some acinus of the meibomian glands. K16 expression significantly expanded in hair follicle, while there was no significant change of K14 and K15 expression in the hair follicle. Demodex-positive tissues were infiltrated with abundant CD11c positive cells and MAC387 positive cells.
Demodex induces hair follicle epithelial cell apoptosis, causes hyper-keratinization and hyper-proliferation of hair follicles, and abnormal keratinizatoin of meibomian gland, as well as inflammation of eyelid tissue.
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