March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Surface Chemistry Study Of The Interactions Of Ofloxacin Eye Ointment With Human Meibum
Author Affiliations & Notes
  • Georgi A. Georgiev
    Biochemistry, Sofia University, Sofia, Bulgaria
  • Norihiko Yokoi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Slavyana Ivanova
    Biochemistry, Sofia University, Sofia, Bulgaria
  • Rumen Krastev
    Biomaterials, NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen, Reutlingen, Germany
  • Zdravko Lalchev
    Biochemistry, Sofia University, Sofia, Bulgaria
  • Footnotes
    Commercial Relationships  Georgi A. Georgiev, None; Norihiko Yokoi, None; Slavyana Ivanova, None; Rumen Krastev, None; Zdravko Lalchev, None
  • Footnotes
    Support  Contract DO 02-280/2008 from the Bulgarian Ministry of Education and Science
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 612. doi:
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      Georgi A. Georgiev, Norihiko Yokoi, Slavyana Ivanova, Rumen Krastev, Zdravko Lalchev; Surface Chemistry Study Of The Interactions Of Ofloxacin Eye Ointment With Human Meibum. Invest. Ophthalmol. Vis. Sci. 2012;53(14):612.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Surface chemistry study of the interactions between Ofloxacin Eye Ointment (Santen Pharmaceutical, Osaka, Japan) and human meibum. Ofloxacin eye ointment (OEO) is the only nonpreserved antibiotic ointment that contains both polar and nonpolar lipids, making it suitable for the treatment of dry eyes.

Methods: : The interactions between OEO and human meibum at the air/water interface were examined in vitro at blink-like compression/expansion of film area by Langmuir surface balance. OEO/meibum films were formed by mixing the compounds in various ratios, from excess of OEO to excess of meibum, and the repulsive or attractive interactions between the ingredients were analyzed by Gibbs additivity rule. The sample’s lateral elasticity and capability to compress and spread during dynamic area changes were evaluated through the surface pressure-area isotherms and isocycles. The lipid films morphology was monitored by Brewster Angle Microscopy.

Results: : Meibum forms thick continuous viscoelastic multilayers with very low (≤ 5 %) surface pressure-area hysteresis during compression/expansion. OEO material properties are very different: it forms thicker rougher films with lower capability to rapidly reorganize and spread during dynamic area changes. When mixed with meibum, OEO climbs over it and forms thick ointment islands layered over tear film lipids. With raise of OEO/meibum ratio, increase of the ingredients aggregation and of the thickness heterogeneity of the mixed films were measured (by Gibbs additivity rule and Brewster Angle Microscopy respectively) accompanied by higher surface pressure-area hysteresis during cycling.

Conclusions: : OEO can not be considered as complete substitute of human meibum, but can serve as good replacement of the non-polar lipids at low OEO/meibum ratios. Surface chemistry based approach is proposed for in vitro molecular scale characterization of artificial meibum substitutes and of their interactions with tear film lipids.

Keywords: cornea: tears/tear film/dry eye • lipids • cornea: basic science 

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