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Motoko Kawashima, Takaaki Inaba, Tetsuya Kawakita, Shigeto Shimmura, Kazuo Tsubota; Effects Of Dietary Fish Oil On Lacrimal Gland In Mice. Invest. Ophthalmol. Vis. Sci. 2012;53(14):622.
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Inflammation of the lacrimal gland plays a significant role in dry eye disease. Omega 3 polyunsaturated fatty acids are the progenitors for novel molecules that play a role in the termination of inflammation and the return of tissues to a state of homeostasis. Fish oil is a source of long-chain omega 3 polyunsaturated fatty acids. Therefore, we investigated dietary fish oil effect on lacrimal gland function in aged mice.
Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into dietary fish oil treatment groups (FO) (n=10) and control groups (CTL) (n=10). After 6 months, tear function was examined under conscious state. After euthanasia, lacrimal glands were subjected to histological examination, and assessment of oxidative stress with 8-hydroxy-2 deoxyguanosine (8-OHdG) and 4-hydroxynonenal (HNE) antibodies. Real time PCR was also performed for MCP-1 and TNF-alpha.
The volume of tear secretion was significantly more in the FO group compared to the CTL group. FO reduced the inflammatory cell infiltration, the concentration of 8-OHdG and the extent of staining with HNE in the lacrimal gland, compared to CTL. In the real time PCR, MCP-1 and TNF-alpha expression were lower in the FO group compared to CTL group.
Fish oil might attenuate oxidative stress damage and suppress inflammatory mediators in the lacrimal gland and preserved lacrimal gland functions.
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