Purchase this article with an account.
kaori ito, Tetsuya Kawakita, Naoko Okada, Masatoshi Hirayama, shigeto shinmura, kazuo tsubota; The Differentiation Potential Of Mouse Lacrimal Gland Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):632.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Lacrimal gland is essential for ocular surface wetness snd maintaining epithelial homeostsasis. We reported mouse lacrimal gland epithelial culture methods in ARVO annual meeting last year. In this study, we analyzed the differentiation potential of those cells.
Three-week-old mice (C57B / 6) were used for this study. Lacrimal glands were removed under anesthesia, enzymatically treated with Collagenase / Hyarulonidase. The cells were seeded in Cnt 07 medium with cholera toxina d subcultured until using. Cells at 56 passages were cultured separately in the following three conditions, usual culture, three-dimensional culture in matrigel, and stimulation confition by TGF-beta10ng/ml. The phenotypic analysis, using antibodies against K14, αSMA, and p63 was performed.
Normal culture condition showed ductal epithelium positive for both K14 and p63. TGF-beta induced alpha-SMA expression. Matrigel culture induced sphere forming.
The myoepithelial cells, and duct cells phenotype were induced from long-term cultured lacrimal gland epithelial cells.
This PDF is available to Subscribers Only