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Keijiro Ishikawa, Shigeo Yoshida, Shintaro Nakao, Yukio Sassa, Mitsuru Arima, Takeshi Kita, Yuji Oshima, Hiroshi Enaida, Toshihiro Kono, Tatsuro Ishibashi; Role Of Periostin In The Pathogenesis Of Epiretinal Membrane With Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2012;53(14):892.
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We previously reported the increased expression of periostin in vitreous and fibrovascular membranes obtained from patients with proliferative diabetic retinopathy (Yoshida et al IOVS 2011). The purpose of this study was to investigate the role of periostin in the pathogenesis of epiretinal membrane with proliferative vitreoretinopathy (PVR).
Vitreous samples and epiretinal membranes were obtained during vitrectomy on patients with PVR. The concentrations of periostin in the vitreous were measured by ELISA, while expression of periostin in PVR membranes was evaluated by immunohistochemical analysis. The expression of periostin in primary human retinal pigment epithelial (RPE) cells stimulated by TGFβ2 was evaluated by realtime RT-PCR, Westen blot, ELISA and immunohistochemical analyses. The effects of periostin on cell proliferation, migration, attachment and contraction were analyzed with BrdU assay, migration assay, MTT assay and collagen gel contraction assay.
The concentration of periostin in the vitreous was significantly higher in patients with PVR than with rhegmatogenous retinal detachment or an epiretinal membrane (p <0.05). The levels of mRNA and protein of periostin were significantly elevated in RPE cells by TGFβ2 stimulation (p <0.01). Immunohistochemical analysis showed co-localization of periostin and α-SMA in cells of PVR membranes and TGFβ2-stimulated RPE cells. Administration of periostin neutralizing antibody or SiRNA significantly suppressed migration and attachment of RPE cells induced by TGFβ2 (p <0.05).
These results indicate that periostin plays a significant role in cell migration and adhesion in the pathogenesis of PVR membranes.
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