April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Expression of TLT-2 in Corneal Tissue and Macrophages after Corneal Transplantation and Association With Immune Privilege of Corneal Allografts
Author Affiliations & Notes
  • Hiroko Taniguchi
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • Hidenori Hase
    Molecular Immunology, Tokyo Medical and Dental University, Tokyo, Japan
  • Hisaya Akiba
    Immunology, Juntendo University School of Medicine, Tokyo, Japan
  • Hideo Yagita
    Immunology, Juntendo University School of Medicine, Tokyo, Japan
  • Miyuki Azuma
    Molecular Immunology, Tokyo Medical and Dental University, Tokyo, Japan
  • Junko Hori
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • Footnotes
    Commercial Relationships  Hiroko Taniguchi, None; Hidenori Hase, None; Hisaya Akiba, None; Hideo Yagita, None; Miyuki Azuma, None; Junko Hori, None
  • Footnotes
    Support  Grant-in-Aid for Scientific Research(C) from the Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1143. doi:
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      Hiroko Taniguchi, Hidenori Hase, Hisaya Akiba, Hideo Yagita, Miyuki Azuma, Junko Hori; Expression of TLT-2 in Corneal Tissue and Macrophages after Corneal Transplantation and Association With Immune Privilege of Corneal Allografts. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1143.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : B7-H3 belongs to the B7 superfamily, a group of molecules that costimulate or down-modulate T-cell responses. We have previously reported that B7-H3 is an essential molecule for acceptance of corneal allografts and induction of allo-specific anterior chamber-associated immune deviation (ACAID). Recently, triggering receptor expressed on myeloid cells-like transcript-2 (TLT-2) has been identified as a B7-H3 receptor. The purpose of the present study is to investigate expression of TLT-2 in the ocular tissue and immune cells, and to elucidate its role on the immune privilege of corneal allografts.

Methods: : Normal corneas of C57BL/6 were transplanted orthotopically into the normal eyes of BALB/c mice. Expression of TLT-2 in normal and in graft-bearing eyes was evaluated by RT-PCR and immunohistochemically by confocal microscopy. In different sets of recipients, anti-TLT-2 monoclonal antibody (mAb) or control rat IgG were administered intraperitoneally, and allograft survival was compared.

Results: : RT-PCR and immunohistochemical studies revealed that there was no expression of TLT-2 in the normal eye. On the other hand, TLT-2 was expressed in the corneal endothelium and epithelium in the allografts. These corneal allografts were infiltrated with TLT-2+CD11b+ cells. In the recipients treated with anti-TLT-2 mAb, all allografts were rejected. The survival of these allografts was significantly shortened in comparison with that of the control group (p<0.05).

Conclusions: : TLT-2 is not expressed in normal ocular tissue, but its expression is up-regulated both in corneal tissue and on macrophages after corneal allo-transplantation. TLT-2 plays a suppressive role in immune response of corneal allografts. TLT-2 expressed in ocular tissue and macrophages within the cornea may contribute to allograft acceptance.

Keywords: immune tolerance/privilege • transplantation • immunomodulation/immunoregulation 
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