Purchase this article with an account.
Kyeong Hwan Kim, Mee Kum Kim, Hyuk Jin Choi, Hyun Ju Lee, So Hee Jeong, Won Ryang Wee, Jin Hak Lee; Effect Of Alpha-gal On The Corneal Xenotransplantation In Gal Knockout Mouse Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1158.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of Galα1-3Galβ1-4GlcNAc-R (α-Gal) on porcine xenocorneal graft survival and production of anti-Gal antibodies in Gal knockout mouse model.
C57BL/6 (B6) and α1,3-galactosyltransferase knockout (GTKO) mice were divided into 5 groups and underwent orthotopically full thickness corneal transplantation as follows (n=4 for each group); (1) group 1: B6 to B6, (2) group 2: fresh porcine posterior corneal lamella to B6, (3) group 3: fresh porcine posterior corneal lamella to GTKO, (4) group 4: decellularized porcine posterior corneal lamella to GTKO, and (5) group 5: B6 to GTKO. Before transplantation, all GTKO recipients were sensitized using intraperitoneal injections of rabbit red blood cells. Median survival time (MST) was compared between groups and serum concentrations of IgG/IgM anti-α-Gal antibodies were evaluated at 1 week, 2 weeks, and 4 weeks.
There was no hyperacute rejection showing MST was around 11 days in all of the groups (p=0.528, Log-Rank test). There were no changes in IgM anti-α-Gal antibodies in all groups, which corresponded well with clinical survival data. While, IgG anti-α-Gal antibody was gradually elevated over time in fresh porcine grafted GTKO recipients.
Our findings suggest that α-Gal is less likely to induce hyperacute rejection but may affect the long term survival via antibody mediated rejection in corneal xenotransplantation.
This PDF is available to Subscribers Only