April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Evidence Of Lymphatic Vessels In The Developing And Young Adult Human Retina and Choroid
Author Affiliations & Notes
  • Tailoi Chan-Ling
    Anatomy, University of Sydney, Sydney, Australia
  • Sam Adamson
    Anatomy, University of Sydney, Sydney, Australia
  • Frank Arfuso
    Anatomy, University of Sydney, Sydney, Australia
  • Jan McColm
    Anatomy, University of Sydney, Sydney, Australia
  • Michele Madigan
    Optometry, University of New South Wales, Sydney, Australia
  • Jane Dahlstrom
    ACT Pathology, Canberra, Australia
  • Mark Koina
    ACT Pathology, Canberra, Australia
  • Elaine Bean
    ACT Pathology, Canberra, Australia
  • Kari Alitalo
    University of Helsinki, Helsinki, Finland
  • Louise Baxter
    Anatomy, University of Sydney, Sydney, Australia
  • Footnotes
    Commercial Relationships  Tailoi Chan-Ling, None; Sam Adamson, None; Frank Arfuso, None; Jan McColm, None; Michele Madigan, None; Jane Dahlstrom, None; Mark Koina, None; Elaine Bean, None; Kari Alitalo, None; Louise Baxter, None
  • Footnotes
    Support  Baxter Charitable Foundation, Rebecca L. Cooper Medical Research Foundation, National Health & Medical Research Council of Australia
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1191. doi:
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      Tailoi Chan-Ling, Sam Adamson, Frank Arfuso, Jan McColm, Michele Madigan, Jane Dahlstrom, Mark Koina, Elaine Bean, Kari Alitalo, Louise Baxter; Evidence Of Lymphatic Vessels In The Developing And Young Adult Human Retina and Choroid. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1191.

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Abstract

Purpose: : To undertake a complete characterisation of the lymphatics in the retina and choroid and human during development and early adulthood.

Methods: : Multiple marker immunohistochemistry with lymphatic endothelial specific markers including PROX1, LYVE-1A, Podoplanin, D-240, VEGF-C and VEGF-R3 of retinal and choroidal wholemounts; transverse sections and TEM. Vascular endothelial specific markers (CD34, CD39) were also applied to the same tissue.

Results: : Lymph vessel growth was found to have a topography of growth that centered around the optic nerve head. Vasculogenesis and angiogenesis appear to precede lymphangiogenesis. Significant structural evidence of lymphatic vessels were found in both the human retina and choroid, but the extent of lymph vasculature was greater in the choroid than the retina. Structural evidence of lymphatic vessels included double-layered vessel walls, lymphatic valves, branch patterns typical of lymphatics, high endothelial cells labelled with lymphatic endothelial specific markers.

Conclusions: : The identification of a significant lymphatic vasculature within the human retina and choroid, and localisation of lymphatic growth factors and receptors necessitates greater understanding of the interaction of the lymphatic vasculature; blood vessels and immune response in the pathogenesis of retinal and choroidal neovascularization.

Keywords: choroid • choroid: neovascularization • age-related macular degeneration 
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