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Alex L. C. Tam, Neeru Gupta, Zhexue Zhang, Yeni H. Yücel; Latanoprost Stimulates Lymphatic Drainage from the Mouse Eye. Invest. Ophthalmol. Vis. Sci. 2012;53(14):1749.
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© ARVO (1962-2015); The Authors (2016-present)
We recently reported a lymphatic outflow pathway from the mouse eye by in vivo imaging of quantum dots (1). Here we determine whether latanoprost stimulates lymphatic drainage from the normal mouse eye.
All procedures adhered to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Under general anaesthesia, 3 μL of quantum dots nanocrystals (QD655) were injected into the left anterior chamber of 11 latanoprost-treated mice and 11 artificial tear-treated control mice. Latanoprost (0.005%) was applied to both eyes 17 hours and 1 hour before in vivo imaging performed with a hyperspectral fluorescence imaging system (Maestro, CRi). Images were captured prior to injection, and at later time points: 5, 20, 40, 70, 120, and 360 min. Following sacrifice at 360 min, imaging was performed to locate QD signals. Neck tissue with signals was removed, and sectioned. QD signal intensity on serial sections was measured using hyperspectral imaging and ImageJ. Immunostains for basement membrane (collagen IV antibody), and Sytox Green were examined by confocal microscopy. Two-sample t-tests were used to compare means of QD signal detection rate (60/time to detection) (hours-1) and means of total QD intensity (log scale) between the two groups.
10 of 11 control mice showed QD signals in the left neck region as early as 120 min (n=3), and 360 min (n=7). In contrast, all latanoprost-treated mice showed left neck signal ranging from 20 min (n=2), 40 min (n=4), 70 min (n=1), to 360 min (n=4) after injection. An increased QD signal detection rate was noted in the latanoprost-treated group compared to controls (1.23±1.06 hours-1 vs. 0.30±0.17 hours-1, mean±SD, P<0.02). Immunofluorescence studies showed QDs confined to the subcapsular region of the left submandibular node in all mice. QD signal intensity was increased in latanoprost-treated mice compared to controls (10.55±1.12 vs. 9.48±1.24, mean±SD, P<0.05).
Latanoprost, a drug commonly used to treat glaucoma, enhances lymphatic drainage from the normal mouse eye. Additional studies in glaucomatous mice are needed to determine whether the action of latanoprost on the lymphatic system contributes to its pressure lowering effects.1. Tam AL, Gupta N, Zhang Z, Yücel YH. Quantum dots trace lymphatic drainage from the mouse eye. Nanotechnology. Oct 21; 22(42):425101, 2011
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