March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Plasmacytoid Dendritic Cells are Modulated During the Immunopathogenesis of Desiccating Stress-Induced Experimental Dry Eye
Author Affiliations & Notes
  • Michael E. Stern
    Biological Sciences, Allergan, Inc, Irvine, California
  • Christopher S. Schaumburg
    Biological Sciences, Allergan, Inc, Irvine, California
  • Jianping Gao
    Biological Sciences, Allergan, Inc, Irvine, California
  • Anh V. Duong
    Biological Sciences, Allergan, Inc, Irvine, California
  • Annie M. Ratanapinta
    Biological Sciences, Allergan, Inc, Irvine, California
  • Virginia L. Calder
    Ocular Biology & Therapeutics, UCL Institute of Ophthalmology, London, United Kingdom
  • Larry A. Wheeler
    Biological Sciences, Allergan, Inc, Irvine, California
  • Jerry Y. Niederkorn
    Ophthalmology, Univ Texas Southwestern Med Ctr, Dallas, Texas
  • Stephen C. Pflugfelder
    Ophthal-Ocular Surf Ctr, Baylor College of Medicine, Houston, Texas
  • Argyrios N. Theofilopoulos
    Immunology, Scripps Research Institute, La Jolla, California
  • Footnotes
    Commercial Relationships  Michael E. Stern, Allergan, Inc. (E); Christopher S. Schaumburg, Allergan, Inc. (E); Jianping Gao, Allergan, Inc. (E); Anh V. Duong, Allergan, Inc. (E); Annie M. Ratanapinta, Allergan, Inc. (E); Virginia L. Calder, Allergan, Inc. (C); Larry A. Wheeler, Allergan, Inc. (E); Jerry Y. Niederkorn, Allergan, Inc. (C); Stephen C. Pflugfelder, Allergan, Inc. (C); Argyrios N. Theofilopoulos, Allergan, Inc. (C)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2327. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Michael E. Stern, Christopher S. Schaumburg, Jianping Gao, Anh V. Duong, Annie M. Ratanapinta, Virginia L. Calder, Larry A. Wheeler, Jerry Y. Niederkorn, Stephen C. Pflugfelder, Argyrios N. Theofilopoulos; Plasmacytoid Dendritic Cells are Modulated During the Immunopathogenesis of Desiccating Stress-Induced Experimental Dry Eye. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2327.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Plasmacytoid dendritic cells (pDC) and derivative type I interferons (IFN-α/β) are important mediators of antiviral immunity. However, type I interferons are also expressed in a variety of autoimmune diseases. Therefore, pDCs were evaluated during the immunopathogenesis of experimental Dry Eye.

Methods: : Female C57BL/6 wild type mice were exposed to desiccating environmental stress (DS; subcutaneous scopolamine injections; humidity <40%; sustained air flow) for 10 days. A combination of flow cytometry, immunohistochemistry and ELISA were used to phenotype pDCs and IFN-α/β within the draining cervical lymph nodes (CLN) and ocular surface tissues over the course of disease.

Results: : Flow cytometric analysis showed elevated frequency of CD11cloPDCA+ pDCs within the draining CLN of Dry Eye mice at day 1 (5.1±1.3%; p=0.04), day 3 (3.1±0.4%; p=0.05), day 5 (5.4±1.0%; p=0.005) and day 10 (4.9±1.8%) compared to naïve control mice (2.2±0.2%). The frequency of CD11cloPDCA+ pDCs was also increased within the ocular surface tissues by day 1 following exposure to DS (6.5±0.1% versus 1.2±0.4%; p=0.0001). Indeed, the higher frequency of pDCs within the CLN correlated with enhanced IFN-α levels by day 1 (2.4±1.2 pg/μg of total protein; p=0.056) and day 5 (3.3±1.4 pg/μg of total protein; p=0.04) of DS relative to control (0.2±1.2 pg/μg of total protein). IFN-α was also increased in the tears of mice with Dry Eye by day 5 of DS (586±57 pg/ml versus 452±35 pg/ml; p=0.03).

Conclusions: : Collectively, these data indicate that pDC are modulated during the development of DS-induced Dry Eye disease.

Keywords: cornea: tears/tear film/dry eye • inflammation • cytokines/chemokines 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×