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yang j. li, Wei Chen; Therapeutic Efficacy Of Trehalose For Treatment Of Murine Dry Eye Induced By Intelligently Controlled Environmental System. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2339.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the beneficial effect of trehalose (TT) instillation and to determine whether the disaccharide operates to alleviate ocular surface damages in a dry eye murine model.
Dry eye was induced in mice using the intelligently controlled environmental system (ICES). After 21 days housed in the ICES without topical treatment, mice were randomly divided into three groups: no eye drops (ICES), treated for three weeks, four times a day with PBS 0.01M 10-µl/eye bilaterally (ICES+PBS) or with TT 87.6 mM 10-µl/eye bilaterally (ICES+TT). A mice group was not exposed to ICES andreceived no treatment to be used as a control group (UT). The ocular surface integrity in each group was evaluated using Oregon Green Dextran- (OGD) and fluorescein-staining. The expression/distribution of occludin, involucrin and SPRR-2 were performed by immunohistology analysis on whole mounted corneas. Hsp70 and MMP-9 expressions were estimated by immunohistology on the corneal tissue sections. The ocular surface inflammation associated to each treatment was estimated by the mRNA quantification of IL-1β, IL-2, IL-6, IL-17 and TNF-α in the conjunctiva by RT-PCR.
The score of OGD staining by the cornea epithelium was significantly lower in ICES+TT group than in ICES and ICES+PBS groups. The corneal epithelium structure appeared, using occludin staining, markedly more homogenous in ICES+TT than in ICES and ICES+PBS groups and showed no desquamating apical epithelial cells. The labelling of involucrin and SPRR-2 on whole mounted corneas revealed an up-regulation of the expression of these proteins in the groups which received no treatment or PBS instillation compared to the ICES+TT group. The immunolabeling of Hsp70 and MMP-9 on tissue sections revealed a marked increase expression in corneal epithelium in response to ICES. The group treated with trehalose showed a similar profile expression of Hsp70 and MMP-9 than the control group (UT). The level expression of mRNA coding to the proinflammatory cytokines IL-1β, IL-2, IL-6, IL-17 and TNF-α were strongly and significantly lower in the conjunctiva of mice exposed to ICES treated with trehalose than in dry eye mice which received no treatment or PBS instillation.
Trehalose application restored the ocular surface integrity in mice with dry eye injuries.
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