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Jun-Sub Choi, Sun-Young Shin, Choun-Ki Joo; Inhibition of corneal lymphangiogenesis by Prox1 siRNA. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2386.
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Lymphangiogenesis in Cornea is induced by various stimuli including infection, inflammation, chemical burn and graft rejection. Angiogenesis and lymphangiogenesis is a common clinical problem. This study was investigated to inhibit lymphangiogenesis by prox1 siRNA. The prox1 is prospero homeobox protein 1 and transcription factor. This protein induces the differentiation of lymphatic endothelial cells.
Induction of lymphatic vessels was stimulated by alkali burning in cornea. 2mm disc with 1N NaOH was applied in central cornea of SD rat for 10 sec. LYVE-1 antibody for lymphatic vessels, and F4/80 antibody for inflammatory cells were used in this study. Prox1 siRNA liposome was treated 2 times per day after burning, topically.
The lymphatic vessels were observed in injured cornea by LYVE-1 immunostaing. And the F4/80 positive cells were observed in this cornea. However, lymphatic vessels and F4/80 positive cells were decreased by prox1 siRNA treatment.
The prox1 siRNA inhibited lymphangiogenesis and inflammation by chemical burning in cornea. These results suggested that prox1 is a regulatory protein for corneal lymphangiogenesis and this prox1 siRNA should be used for inhibition of corneal lymphangiogenesis.
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