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Alejandro Navas, Rodrigo Bolaños-Jimenez, Hilen Bravo-Briseño, Carlos Estrada, Alexandra Luna-Angulo, F J. Estrada, Yonathan Garfias; CCR3 Expression in Corneal Neovascularization. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2392.
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© ARVO (1962-2015); The Authors (2016-present)
It has shown that corneal stress such as dry eye syndrome and mechanical corneal injury induce the expression of the eotaxin receptor CCR3 (CD193) transcript. By the other hand, it has also demonstrated that CCR3 protein is specifically expressed in choroidal neovascular endothelial cells in humans with age-related macular degeneration. The aim of this study is to determine whether there is an induction of the expression of CCR3 protein in a corneal neovascularization murine model.
After complete epithelial removal, 2 mm diameter alkali (1N NaOH) burns were performed in the right cornea of BALB/c mice, according to the ARVO guidelines for animal use for research, in order to generate corneal neovascularization. Clinical neovascularization parameters were recorded from day 1 to day 12 after corneal injury. The CCR3 transcript expression was identified by means of RT-PCR in corneal neovascularized specimens. The CCR3 protein presence in the corneal tissue was identified using indirect immunofluorescence.
Clinical neovascularization was time dependent, observing a maximal corneal neovasculzarization at day 5 with a vascularization regression at day 12 after chemical injury. CCR3 mRNA expression was exclusively expressed in neovascularized corneas in comparison to the non-neovascularized (control) corneas in which CCR3 mRNA expression was absent; in both cases actin mRNA was similarly expressed. When immunofluorescence assays were performed, we were able to corroborate the CCR3 presence in the neovascularized corneas; interestingly, CCR3 co-localized with CD31. The non-neovascularized corneas were negative to both markers.
Taken together these results suggest that eotaxin receptor CCR3 might play a noteworthy role in corneal neovascularization and could be used as a target in this significant ocular affection.
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