Purchase this article with an account.
Thomas O. Muldoon, Richard B. Rosen, Dan N. Hu, Men Chen, Howard Savage, Steven A. McCormick; Protective Effects Of Zeaxanthin On Retinal Pigment Epithelial Cells Subjected To Methylglyoxal Damage. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2435.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Methylglyoxal (MG) is the intermediate product of glycation and is the precursor of advanced glycation endproducts (AGEs). MG itself and its metabolic products (AGEs) are toxic to the cells. Excessive accumulation of MG due to aging or hyperglycemia plays a role in the pathogenesis of various ocular diseases, e.g. age-related macular degeneration and diabetic retinopathy. The present study investigated the protective effects of zeaxanthin on retinal pigment epithelial (RPE) cells against MG damage.
The toxic effects of MG and the protective effects of zeaxanthin on the cell viability were tested in two human RPE cell lines (ARPE19 and a primary culture of RPE cells from donor eye) using the MTT test. Cells were seeded into 96 well plates. Zeaxanthin was added to the medium at various concentrations. MG (0.3 mM) was added 2 hr later and cultured for another 24 hr. Cells cultured without any additive and cells cultured with MG alone were used as the negative and positive controls, respectively. All groups were tested in triplicate.
MG at 3 mM caused a decrease of 50 to 59% of cell viability of cultured RPE cells. Pretreatment of zeaxanthin at 10-100 μM significantly protected RPE cells against MG damage. Zeaxanthin at 10, 30 and 100 μM increased the cell viability of RPE cells to 124%, 142% and 152% of cells treated with MG alone in ARPE cells, respectively (P < 0.05 in all groups). Zeaxanthin at 10, 30 and 100 μM increased the cell viability to 120%, 130% and 148% of cells treated with MG alone in primary culture of RPE cells (P < 0.05 in all groups). However, cell viability of cells treated with zeaxanthin and MG was still significantly lower than that in cells not treated with MG (P < 0.05).
Zeaxanthin at 10-100 μM levels has a partial and significant protective effect on the RPE cells subjected to MG damage in vitro.
This PDF is available to Subscribers Only