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Zeljka Smit-McBride, Saadia Rashid, Alfred K. Yu, Susanna S. Park, Leonard M. Hjelmeland, David G. Telander, Lawrence S. Morse; Analysis And Comparison Of Human Circulatory MicroRNA Profiles Of Aqueous And Vitreous Humour From Normal Eyes vs. Those With Diabetic Retinopathy or AMD. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2438.
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To investigate aqueous and vitreous humour microRNA profiles and to explore the difference and similarities of microRNA species in normal eyes vs. diabetic retinopathy or AMD patients.
Samples (12) were collected during standard of the care vitreoretinal surgery - 3 control aqueous, 3 control vitreous, 2 diabetic retinopathy (DR) aqueous, 2 DR vitreous, and 2 wet AMD aqueous. Controls were 2 macular puckers and 1 displaced lens surgery sample. Samples were preserved in RNAprotect, and stored at -20oC. MicroRNAs were isolated using QIAgen microRNeasy kit. MicroRNA samples were quantified using a Nanodrop, labeled with FlashTag kit and profiled on Affymetrix GeneChip miRNA 2.0 microarrays. Data analysis was done using miRNAQCtool (Affymetrix) and IPA- Ingenuity Pathway Analysis software.
Comparison of circulatory microRNA population of aqueous and vitreous humour showed that out of a total of 847 miRNA probes on the Chip, 25 were present in both aqueous and vitreous samples, an additional 11 being unique for aqueous samples, and 7 unique for vitreous. The most abundant common microRNAs are members of the families hsa miR-218 (regulation of vascular patterning and vascular guidance cues), hsa-miR-193 (differentiation of telocytes, stromal cells between blood vessels and neurons, with a role in intercellular signaling), and miR-let-7 (regulation of retinal Muller glia dedifferentiation and retinal regeneration in teleosts). Comparison of aqueous and vitreous showed a very similar abundance for a set of common miRNAs, and there are certain circulatory microRNAs that are detected only in diabetic retinopathy and a different set of microRNAs detected only in AMD patients.
The comparative profiling of circulatory microRNAs in the aqueous and vitreous humour of normal eyes vs. those with diabetic retinopathy or AMD shows that these molecular species are present in all samples with variability related to the presence of either diabetic retinopathy or AMD. These results offer a rationale for further studies on the potential use of ocular fluids for diagnostic or ultimately therapeutic approaches involving micro RNAs.
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