March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Decreased Erg Response In Mice With CNV
Author Affiliations & Notes
  • Irina Semkova
    Ophthalmology, Charite, University Medicine Berlin, Berlin, Germany
  • Sergei Skosyrski
    Ophthalmology, Charite, University Medicine Berlin, Berlin, Germany
  • Michael Dutescu
    Ophthalmology, Charite, University Medicine Berlin, Berlin, Germany
  • Norbert Kociok
    Ophthalmology, Charite, University Medicine Berlin, Berlin, Germany
  • Antonia M. Joussen
    Ophthalmology, Charite, University Medicine Berlin, Berlin, Germany
  • Footnotes
    Commercial Relationships  Irina Semkova, None; Sergei Skosyrski, None; Michael Dutescu, None; Norbert Kociok, None; Antonia M. Joussen, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2445. doi:
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    • Get Citation

      Irina Semkova, Sergei Skosyrski, Michael Dutescu, Norbert Kociok, Antonia M. Joussen; Decreased Erg Response In Mice With CNV. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2445.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To test the feasibility of recording single and multifocal electroretinograms (ERGs) from the mouse eyes with developed CNV.

Methods: : ERGs were recorded 2 weeks after laser treatment of C57Bl/6 mice and their corresponding controls. Multifocal ERGs were recorded using an array of equal-sized hexagons. Local response isolation was evaluated by comparing multifocal responses to full-field ERGs and by mapping local defects in laser-treated mice. Full-field ERG with scotopic and photopic records was also performed. CNV development was identified by angiography and OCT.

Results: : Two weeks after laser treatment (5 spots per eye) CNV areas with fluorescein leakage were observed. OCT showed breakdown of the Bruch’s membrane and severe CNV lesions. Full-field ERG showed significantly decreased response in scotopic A- and B-wave. Moderate, but still significant reduction of photopic B-wave response was also observed in laser-treated mice. The most decreased response compared to the controls was the C-wave response. ERG responses were also observed by multifocal ERG.

Conclusions: : ERG responses was significantly decreased in eyes with developed CNV. The usage of the full-field and multifocal technique will be useful in characterizing local retinal/RPE defects created with laser treatment.

Keywords: age-related macular degeneration • retina • neovascularization 
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