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Sandeep Kumar, Lizhen Ren, John Danias, Lampros Panagis; Effects of Triamcinolone on the Outflow Facility in C57BL/6J Mouse Eyes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2483.
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To determine the effects of steroids on outflow facility in mice.
C57BL/6 mice were injected under the conjunctiva with 10ul of 40ug/ml Triamcinolone acetonide suspension. Uninjected eyes of other C57BL/6J mice were used as controls to ensure absence of steroid effects on contralateral eyes. Three weeks after the injection, the animals were euthanized and their eyes were used for outflow facility measurement. Two eyes developed severe infection and the animals were euthanized. Outflow facility was determined using simultaneous pressure and flow measurements. Flow at constant predetermined levels was achieved with a syringe pump while an inline pressure transducer was used to measure intraocular pressure. Outflow facility in steroid treated eyes was compared to that of control eyes using T-test.
C57BL/6J eyes had outflow facilities ranging between 0.006 and 0.0083 ul/min/mmHg in all eyes studied (n=6). Outflow facility in steroid treated mice eyes ranged between 0.0013 and 0.0027 ul/min/mmHg in all eyes studied (n=7). Difference in outflow facility between control and steroid treated eyes was statistically significant (t-test, p<0.001).
Steroid treatment affects the outflow facility of mice. However, periocular steroid injection can cause severe infections in a small percentage of animals.
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