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Momoko Shibasaki, Ji-Ae Ko, Jouji Takenaka, Tai-ichiro Chikama, Yoshiaki Kiuchi; Analysis of Molecular Mechanisms on scarring formation after Experimental Glaucoma Filtration and Implant Surgery. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2504.
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Glaucoma filtering surgery usually fails because of post surgical scarring, a process in which fibroblasts play a prominent role. To examine the molecular mechanism in post surgical scarring (fibrosis), we have now investigated the factors with sclerostomy and implant surgery in rat models.
In a randomized, prospective study. In 3 Sprague-Dawley rats, 6 eyes, glaucoma filtration surgery was performed. On one eye, sclerostomy under conjunctival flap with MMC was performed and on the fellow eye a silicone cannula through a scleral tunnel under a conjunctival flap was introduced. The animals were killed 10 days after surgery (starting to break the bleb), we have harvested the tenon fibroblasts, surrounding the bleb. Tenon fibroblasts were cultured, and were examined the expression of MMPs by reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot analyses. Also, we have examined the culture supernatant using multiplex arrays.
Immunoblot and RT-PCR analysis showed that amounts of MMP-1, 3 were increased in scarring after implant surgery. But, others MMP (MMP-2, 9, 13) were not changed in scarring after both surgery. And, we have known the secreted IL-6 was increased in culture medium of tenon fibroblasts after sclerostomy than implant.
These results suggest that MMP-1, 3 may play an important role in scarring after glaucoma surgery. And, it will be possible that cytokine IL-6 regulates the expression of MMP-1, 3 during scar formation.
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