March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Qualitative Analysis Of Intraretinal Pigment Using en-face SDOCT Imaging
Author Affiliations & Notes
  • Jyoti R. Dugar
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Carlos Alexandre A. Garcia Filho
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Zohar Yehoshua
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Giovanni Gregori
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • William Feuer
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Philip J. Rosenfeld
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Footnotes
    Commercial Relationships  Jyoti R. Dugar, None; Carlos Alexandre A. Garcia Filho, Carl Zeiss Meditec, Inc. (F); Zohar Yehoshua, Carl Zeiss Meditec,Inc. (F); Giovanni Gregori, Carl Zeiss Meditec, Inc. (F, P); William Feuer, Alexion (F); Philip J. Rosenfeld, Alexion (F), Carl Zeiss Meditec, Inc. (F, R)
  • Footnotes
    Support  Alexion Phamaceuticals; Macula Vision Research Foundation; NIH center core grant P30EY014801; Research to Prevent Blindness; Department of Defense (W81XWH-09-1-0675); Grant from Carl Zeiss Meditec Inc
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2667. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jyoti R. Dugar, Carlos Alexandre A. Garcia Filho, Zohar Yehoshua, Giovanni Gregori, William Feuer, Philip J. Rosenfeld; Qualitative Analysis Of Intraretinal Pigment Using en-face SDOCT Imaging. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2667.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

To describe a new method for identifying the presence and distribution of intraretinal pigment clumps derived from retinal pigment epithelial cell migration using SDOCT imaging.


Patients with the diagnosis of age-related macular degeneration (AMD) were enrolled in a prospective SDOCT imaging study. Patients were followed for a minimum of 6 months. Eyes were imaged using the 200x200 A-scan raster pattern (Cirrus HD-OCT, Carl Zeiss Meditec)) centered on the fovea. En face images were obtained by summing the OCT intensities in a region bounded by the retinal pigment epithelium (RPE) segmentation line and a line parallel to the RPE segmentation line but located at distances of 40 µm, 60 µm, 90 µm and 120 µm above it. A second set of en-face images was obtained by translating each of these regions upwards by 20 µm.


A total of 16 eyes with drusenoid detachments of the RPE were included in this study. Mean follow-up was 23.8 months. The drusenoid RPE detachments persisted in 8 eyes (50%), evolved to geographic atrophy (GA) in 5 eyes (31.25%), developed CNV in 2 eyes, and resolved without formation of GA or CNV in 1 eye. During follow-up, retinal pigment was observed on exam, color fundus imaging, and SDOCT imaging in 9 out of 16 eyes (56.3%). Intraretinal pigment clumping was observed in all 5 eyes developing GA, in 2 out of 8 eyes with persistent RPE detachments, in one eye developing CNV, and in the one eye with resolution of the PED in the absence of obvious disease progression. Intra-retinal pigment was observed in all en face images of the same patient. Images obtained by shifting the RPE contour by 20 µm and using a slab thickness of 40 µm and 60 µm provided the best visualization of the pigment.


SDOCT en face imaging can provide qualitative assessment of pigment clumps within the central macula by using a slab based on the RPE segmentation contour but shifted 20 µm above the actual RPE boundary. The exclusion of the RPE and the photoreceptor IS/OS junction from these images may be responsible for the better contrast and identification of the pigment clumps.

Keywords: age-related macular degeneration • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.