Purchase this article with an account.
Sung-Hye Kim, Da-Young Ko, Jenna Burton, Cheng-Rong Yu, Charles Egwuagu; Resveratrol: Potential Drug For Treating Uveitis And Other Autoimmune Disease. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2761.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Experimental autoimmune uveitis (EAU) is a T cell mediated inflammatory disease that shares essential pathological features with human uveitis and serves as a mouse model of posterior uveitis. Th17 and Th1 cells have been implicated in the etiology of uveitis and suppressing the expansion of these CD4+ T cell subsets during uveitis may be therapeutically beneficial. Resveratrol is an antioxidant polyphenols contained in red wine and grape skin and has been shown to possess anti-inflammatory properties. In this study, we investigated whether Resveratrol might inhibit the expansion of uveitogenic T cells and ameliorate EAU.
The naïve CD4 T cells from C57BL/6 mice were isolated and cultured under Th1 or Th17 polarization condition in the presence or absence of Resveratrol. Cellular proliferation/expansion was determined by the antigen-induced CD154 expression assay, 3H-thymidine incorporation or CFSE labeling assay. Gene expression was analyzed by RT-PCR and quantified by qPCR. Intracellular cytokine expression was detected by FACS analysis and effects of Resveratrol on cell survival was determined by Annexin-V staining assay. EAU was induced by adoptive transfer of IRBP-specific pathogenic T cells and effects of Resveratrol on development and severity of EAU was monitored by fundoscopy or histopathology.
Resveratrol suppressed the differentiation of Th17 and Th1 cells and inhibited the expansion of uveitogenic Th17 cells during EAU. Resveratrol inhibited adoptive transfer of uveitis to syngeneic mice.
Resveratrol could potentially be a safe and effective drug for treating uveitis and other autoimmune disease.
This PDF is available to Subscribers Only