March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Pathogenicity of Staphylococcus epidermidis in bacterial endophthalmitis
Author Affiliations & Notes
  • Sujata Prasad
    Ocular Microbiology,
    ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, India
  • Niranjan Nayak
    Ocular Microbiology,
    ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, India
  • Gita Satpathy
    Ocular Microbiology,
    ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, India
  • T. C. Nag
    Anatomy,
    ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, India
  • Pradeep Venketesh
    Uvea & Retina Services,
    ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, India
  • Footnotes
    Commercial Relationships  Sujata Prasad, None; Niranjan Nayak, None; Gita Satpathy, None; T. C. Nag, None; Pradeep Venketesh, None
  • Footnotes
    Support  Indian Council of Medical Research Grant, India
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2774. doi:
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    • Get Citation

      Sujata Prasad, Niranjan Nayak, Gita Satpathy, T. C. Nag, Pradeep Venketesh; Pathogenicity of Staphylococcus epidermidis in bacterial endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2774.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Staphylococcus epidermidis a normal commensal of the eye is regarded as one of the common etiological agents of bacterial endophthalmitis.The purpose of the study was to determine the pathogenicity of Staphylococcus epidermidis isolates from cases of post-operative endophthalmitis

Methods: : A total of 47 isolates were obtained from vitreous sample of patients with post operative endophthalmitis. Biofilm producing ability was studied both phenotypically (by quantitative adherence test) and genotypicaly by polymerase chain reaction (PCR) amplification of ica gene. Scanning electron microscopy (SEM) was employed to study the biofilms on intra ocular lenses.

Results: : Out of the 47 isolates, 23 (48.9%) were adherent and 24 (51.0%) were non adherent. PCR showed that 16 (34.0%) isolates contained the ica locus whereas 31(65.9%) isolates did not possess the ica gene. SEM picture revealed that isolates carrying ica gene had formed biofilms on intra ocular lens.

Conclusions: : This study provides the information that the isolates carrying ica gene were found to be adherent and biofilm production could be incriminated as a virulence marker for Staphylococcus epidermidis causing post operative endophthalmitis.Our results also suggest that PCR can be a sensitive and rapid test to detect biofilm forming ability of the organism in post operative endophthalmitis which is well known to be a vision threatening complication of surgery.

Keywords: endophthalmitis • Staphylococcus • intraocular lens 
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