March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
3D Quantification of Laminar Microarchitecture (LMA) in Normal Non-Human Primate (NHP) Eyes
Author Affiliations & Notes
  • Howard Lockwood
    Optic Nerve Head Research Laboratory, Devers Eye Institute, Portland, Oregon
  • Juan Reynaud
    Optic Nerve Head Research Laboratory, Devers Eye Institute, Portland, Oregon
  • Jonathan Grimm
    Dept. of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA;, UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Pittsburgh, Pennsylvania
  • Hongli Yang
    Optic Nerve Head Research Laboratory, Devers Eye Institute, Portland, Oregon
  • Stuart Gardiner
    Optic Nerve Head Research Laboratory, Devers Eye Institute, Portland, Oregon
  • J Crawford Downs
    Optic Nerve Head Research Laboratory, Devers Eye Institute, Portland, Oregon
  • Claude Burgoyne
    Optic Nerve Head Research Laboratory, Devers Eye Institute, Portland, Oregon
  • Footnotes
    Commercial Relationships  Howard Lockwood, None; Juan Reynaud, None; Jonathan Grimm, None; Hongli Yang, None; Stuart Gardiner, None; J Crawford Downs, None; Claude Burgoyne, None
  • Footnotes
    Support  NIH Grant EY-
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2825. doi:
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    • Get Citation

      Howard Lockwood, Juan Reynaud, Jonathan Grimm, Hongli Yang, Stuart Gardiner, J Crawford Downs, Claude Burgoyne; 3D Quantification of Laminar Microarchitecture (LMA) in Normal Non-Human Primate (NHP) Eyes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2825.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To calculate and characterize the regional distributions of lamina cribrosa beam diameter (BD), pore diameter (PD), connective tissue volume (CTV) and connective tissue volume fraction (CTVF) from high-resolution, digital 3D optic nerve head (ONH) reconstructions of 17 normal NHP eyes.

 
Methods:
 

The ONH reconstructions were 3D-aligned to a clinical photograph or confocal scanning laser tomographic (CSLT) image and the lamina cribrosa was 3D segmented [IEEE Trans Med Imag, 2006; 25:245-255]. Each voxel was assigned a "diameter" value based upon the largest sphere that fit inside the "beam" or "pore" in which it was contained. CTV was defined to be the total volume of beam voxels. CTVF was defined to be the ratio of CTV to total volume (pore + beam voxels). For each ONH, BD, PD, CTV and CTVF data were digitally converted to right eye orientation and mapped to a cylinder of radius 1.5 mm and 120 μm thickness. Data were regionalized by clock hour using the BMO-centroid to foveal axis as the horizontal midline allowing direct comparisons between eyes with varying laminar and scleral geometries.

 
Results:
 

Regional BD and PD did not deviate significantly from a normal distribution according to the Shapiro-Wilk test. Generalized estimating equation models were constructed using the distance from the horizontal to determine parameter variability nearer the superior and inferior poles. Beams were slightly thinner within the vertical compared to the horizontal clock hours (p=0.05, see above).

 
Conclusions:
 

These data expand our previous characterizations of normal NHP LMA [J. Grimm et al, ARVO, 2007; Roberts, et al IOVS. 2009;50:681-690] to include laminar BD, PD, CTV and CTVF in 17 normal eyes. The regional distributions of normal NHP laminar BD, PD, CTV and CTVF are eye-specific (data not shown). However pooled data for all 17 eyes (see Figure) suggest that on average, BD is greatest temporally, PD is greatest inferior and superior temporally, CTV is greatest superiorly and inferiorly, and laminar connective tissue density (CTVF) is greatest temporally.  

 
Keywords: imaging/image analysis: non-clinical • lamina cribrosa • optic nerve 
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