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ARACELI ROJAS DÍAZ, Rojas Diaz Joel, Ramirez Estudillo Juan Abel, Levine Berebichez Arthur, López Espinosa Nadia L., Bautista de Lucio Victor Manuel; Response Of Choroidal Neovascularization In Animal Models With Use Of Amniotic Membrane.. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2959.
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o Identify the response of choroidal neovascularization with the use of amniotic membrane. establishing a reproducible model of laser choroidal neovasculariación, identifying in each group and variable expression of proangiogenic factors.
A prospective, observational, longitudinal, experimental, interventional, analytical and descriptive study was done. Four groups were created a market consisting of three rabbits, the first with placebo intravitreal injection, the second intravitreal injection of lyophilized amniotic membrane, the third was a model of choroidal neovacularización created with laser, and the fourth a model of choroidal neovascularization treated with intravitreal amniotic membrane, studies clinical, fluorangiographyc and determination of pro and anti-angiogenic factors by chemiluminescence.
A total of 15 rabbits 3 of which were used to create the model of choroidal neovascularization where the average laser power used was 2 W with exposure of 500 ms and spot diameter of 100 microns and the remaining 12 were divided into 4 groups described above, the rabbits in group 4 who had choroidal neovascularization with application of amniotic membrane had better clinical response and fluorangiography compared to group 3 received no treatment with amniotic membrane, the expression of proangiogenic factors are overexpressed in the group untreated and decreased in the group treated with amniotic membrane.
There were clinical differences between the group not treated with amniotic membrane and treated with the same, being better in the group treated with amniotic membrane, There are over expression of VEGF and proangiogenic factors in the group with choroidal neovascularization, decreasing in the group treated with amniotic membrane. Model was obtained reproducible neovascularization with laser.
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