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Norbert Kociok, Sergej Skosyrski, Irina Semkova, Antonia M. Joussen; Laser Induced-choroidal Neovascularisation Enhances The Mrna Expression Of Sphingosine-1-phophate Receptors In The Retina. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3013.
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Sphingosine-1-phophate (S1P) is a multifunctional lipid molecule that stimulates endothelial cell migration and proliferation via the S1P family of G protein-coupled receptors (S1P(1-5)R). Activation of the sphingosine 1-phosphate receptor 1 (S1P(1)R) protects against renal ischemia-reperfusion injury and inflammation. Recently, the inhibition of S1P was considered as a therapeutic treatment of AMD patients. Here we analyze the mRNA expression of S1P(1)R and S1P(3)R in a mice model of choroidal neovascularisation.
Choroidal neovascularisation was induced in C57Bl/6-mice by disrupting the Bruch’s membrane by laser treatment. 14 days after laser treatment the retina was removed, the RNA was isolated and a Real Time RT-PCR analysis for S1P(1)R and S1P(3)R was performed.
A clear scare formation in the choroid was detected by angiography in laser treated eyes. In the retinas of laser treated mice the mRNA expression of S1P(1)R was enhanced between 26.6 ± 5.6 and 4.3 ± 1.4 when compared to not treated control eyes. The expression of S1P(3)R in these retinas was enhanced between 5.1± 0.9 and 20.5 ± 2.5.
The enhanced mRNA expression if these S1P receptors in the mouse retina after laser treatment was shown. Choroidal neovascularisation has an effect on the sphingosine-1-phosphate receptor gene expression in the retina. This pathological effect on the retina may be attenuated by blocking the action of sphingosine-1-phosphate or its receptors.
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