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Millena G. Bittencourt, Yasir J. Sepah, Mohhamed Ibrahim, Raafay Sophie, Owhofasa Agbedia, Abeer Akhtar, Hyun S. Jang, Jithin Yohannan, Diana V. Do, Quan D. Nguyen; Comparison of Fundus Autofluorescence Images Obtained by Two Different Wavelengths of Light in Normals, Myopic Subjects, and Patients with Retinochoroidopathy. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3108.
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To evaluate if different wavelengths of light produce different patterns of fundus autofluorescence (FAF) images in normals, myopic subjects, and patients with retinchoroidopathy (RC).
Normals, myopics and patients with RC were enrolled. FAF images were acquired from each subject using blue light (BL) of 488nm Spectralis® (Heidelberg CA), and green light (GL) of 532nm, 200TxTM (OPTOS, UK). The pairs of BL FAF and GL FAF were analyzed using Photoshop (Adobe Systems, CA). The grey scale values (range: 1 to 255) of the same points in both FAF images were measured. The optic disc was used as standard for absence of fluorescence, as the lowest amount of fluorescence is seen in the optic disc in BL FAF. Thus, the disc value was compared with those values in other retinal areas. The difference between values in the area of interest and in the optic disc was calculated and then used to compare FAF images.
Among the 51 pairs of images analyzed, 8 normal subjects (15 eyes) contributed 24 pairs of BL and GL FAF images, 4 subjects with high myopia (8 eyes) contributed 10 pairs of FAF images, and 12 patients with various types of RC (23 eyes) contributed 27 pairs of images. Myopics had a mean refractive error of -8.40 Diopters (D) (SD ±2.71D). The results are summarized in Table 1.
The gray scale interval visualized on images acquired by 200Tx™ is greater than the one employed by Spectralis®. Acquired FAF images differ between Spectralis® and 200Tx™ in normal subjects and myopics, and therefore cannot be used interchangeably. However, both devices appear to agree on the changes seen among patients with RC. Further analyses are indicated to confirm if the images from Spectralis® and 200Tx™ may provide similar or different AF characterization of diseases.
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