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Preejith P. Vachali, Aihua Liu, Binxing Li, Zhengqing Shen, Brian Besch, Mike Black, Ryan Terry, Paul S. Bernstein; Optimization Of The Uptake And Bioavailability Of Water-based Nano-dispersions Of Zeaxanthin In C57BL/6 Mice. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3374.
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Dietary lutein and zeaxanthin, the two principal carotenoids in the human eye, can play a protective role against human ocular diseases such as age-related macular degeneration and cataract. Carotenoids are lipophilic compounds with relatively low oral bioavailability in mouse models. In this study, we evaluated the absorption and bioavailability of zeaxanthin-sucrose monolaurate (SML) and zeaxanthin-Captisol nano-dispersed in aqueous media. These carotenoid formulations were given to transgenic mice, with a specific zeaxanthin-binding-protein (human GSTP1) over expressed in their retinas.
C57BL/6 mice, (13-15 weeks old) were used. The experimental groups (10 each) GSTP1 transgenic and wild type received zeaxanthin-Captisol (12.5 mg/day/mouse) by daily gavage or the zeaxanthin- SML (0.140 mg/day/mouse) in drinking water. The control group (3 wild type mice) received Captisol or SML alone. The tissues were harvested after 4 weeks of feeding, and the samples were analyzed using HPLC.
Results are summarized in the attached table.
While the zeaxanthin-Captisol method administered 10 times more carotenoid than the zeaxanthin-SML method, the resulting serum levels did not vary much between the dosages, and we were unable to detect any zeaxanthin in the retina. The zeaxanthin-SML method seems to be a promising method of delivery, as the amount of carotenoids delivered per day is closer to the physiological level an adult human would receive via supplementation, and it can be delivered conveniently in drinking water. We are currently optimizing various conditions of zeaxanthin delivery to achieve a higher level of serum uptake and improved delivery into the retina of the GSTP1 transgenic mice.
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