Purchase this article with an account.
Diego Garcia-Ayuso, Manuel Salinas-Navarro, Marta Agudo-Barriuso, Francisco Manuel Nadal-Nicolás, Manuel Jiménez-López, Caridad Galindo-Romero, Manuel Vidal-Sanz, María Paz Villegas-Pérez; Sectorial Loss Of Retinal Ganglion Cells In The RCS Rat. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3487.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate whether the sectorial loss of retinal ganglion cells (RGC) observed in previous studies of our laboratory in the dystrophic Royal College of Surgeons (RCS) rat strain (Villegas-Pérez et al., J Comp Neurol 1998;392: 58-77) is due to an axonal transport deficit problem or to retinal ganglion cell death.
Dystrophic (rdy–/p+) and non-dystrophic (rdy+/p+) pigmented RCS rats with ages ranging from 12 to 20 months were used for this study. RGCs were identified using Fluoro-Gold (FG) tracing from the Superior Collicullus (SC), to label RGCs with a competent axonal transport and Brn3a immnunodetection, to detect all RGCs. Retinas were processed as whole mounts and examined by fluorescence microscopy. Reconstructions of the whole mounts were made using Image-Pro Plus 5.0 for Windows®. FG-labelled and Brn3a positive RGCs were automatically identified and counted in each retina using previously described methods (Salinas-Navarro et al., Vision Res. 2009;49: 115-126, Nadal-Nicolás et al., IOVS 2009;50: 3860-3868).
Dystrophic retinas showed with age wedge-shaped sectors devoid of both FG labelling and Brn3a detection. These sectors were observed first in the ventral retina and later spread dorsally. The number of both FG-labelled and Brn3a positive RGCs declined with age. However, at 20 months of age there were significantly higher numbers of Brn3a positive than of FG-labelled RGCs. Taken together, these data indicate that the sectors are due to RGC death, which is preceded by an axonal transport deficit.
Sectorial RGC loss in the dystrophic RCS rat retina is due to an axonal transport deficit that ultimately causes RGC death.
This PDF is available to Subscribers Only