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Joyce L. Covre, Babyla G. Monteiro, Priscila C. Cristovam, Renata R. Loureiro, Irina Kerkis, Jose Alvaro P. Gomes; Immature Dental Pulp Stem Cells and better way to differentiate into corneal epithelium cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3508.
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Evaluate the better culture medium to induce human Immature Dental Pulp Stem Cells (hIDPSC)to differentiate into corneal epithelium cells.
hIDPSC were isolated and characterized according to Kerkis et al. (2006). It were selected four cultures media: Epilife, Knockout DMEM, KSFM and SHEM. All the cultures were mantained per 7 days, and the media were changed every day. For each specific culture medium tested were made in triplicate. After 7 days the samples were fixed and prepared for immunocitochemistry analysis. To confirm the differentiation of hIDPSC we used K3/12 .
Our data showed that samples cultivated with Knockout DMEM and SHEM reacted positively to K3/12, indicating that the cells were able to differentiate into corneal epithelium cells in 7 days. The hIDPSC cultivated in Epilife and KSFM media didn't show positive reaction .
According to our data the Knockout DMEM and SHEM media were better than Epilife and KSFM for inducing differentiation into corneal epithelium cells.
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