March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Effects Of Beta-glucan on Corneal Inflammation by Alkali Burn Model
Author Affiliations & Notes
  • Choun-Ki Joo
    Catholic Institutes of Visual Science,
    Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
  • Jun-Sub Choi
    Ophthalmology & Visual Science,
    Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Choun-Ki Joo, None; Jun-Sub Choi, None
  • Footnotes
    Support  This study was supported by a grant of the Korean Health Technology R & D Project, Ministry for Health & Welfare, Republic of Korea. (A092258)
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3531. doi:
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      Choun-Ki Joo, Jun-Sub Choi; Effects Of Beta-glucan on Corneal Inflammation by Alkali Burn Model. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3531.

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Abstract

Purpose: : To investigate the effect of topical β-glucan on corneal alkali burn model

Methods: : Immortalized human corneal epithelial cells (HCECs) were cultured in media with different concentrations of Laminarin (β-1, 3- and β-1, 6-glucans) solution, β-glucan (β-1, 3-glucan) solution, β-glucan-carboxylmethyl cellulose (CMC), β-glucan-hyaluronic acid (HA). Wound healing assays were conducted via the manual scraping of cell cultured plate after 24hrs. And fifty eyes from 50 Sprague-Dawley male rats were used for alkali burn model. After alkali injuries using 1 N NaOH, the control group (n=10) received topical PBS four times a day for 2 days. The other study groups received topical Laminarin (n=10), β-glucan (n=10), β-glucan-CMC (n=10), and β-glucan-HA (n=10). Using slit lamp biomicroscopy, injured corneas were evaluated and tissue sections were analyzed histologically for cellular infiltration

Results: : The significant differences in wound healing rates were noted in the Laminarin, β-glucan groups, and positive control cells, as compared to negative control (p<0.05, respectively). And the wound healing rate of the β-glucan groups was significantly higher than that of the Laminarin group (p<0.05). In the rat wound healing model, the significant differences in wound healing rates were significantly noted in the Laminarin, and β-glucan groups, as compared to the control control (p<0.0001, respectively). In light microscopic findings, the corneal thickness was increased and many polymorphonuclear leukocytes infiltrated in the corneal stroma in the control group

Conclusions: : The topical β-glucan solution has beneficial effect on inflammatory corneal wound healing in vitro and in vivo

Keywords: wound healing 
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