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Mercede Majdi, Asadolah Movahedan, Hungyu Ying, Hossein M. Sagha, Behrad Y. Milani, Maryam A. Shafiq, Robert M. Lavker, Beatrice Y. Yue, Ali R. Djalilian; The Role Of Notch Signaling Pathway In Corneal Epithelial Cell Migration. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3544.
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© ARVO (1962-2015); The Authors (2016-present)
To elucidate the role of Notch signaling in corneal epithelial cell migration, by examining the effect of endogenous activation of Notch in-vitro.
Human corneal epithelial cell line (SV40) was transiently transfected with intracellular NotchIC-GFP or GFP carrier plasmid. Individual cell migration was compared between control cells (GFP trasfected) and Notch activated cells (NotchIC-GFP transfected) in a scratch assay followed by live microscopy using Spinning disc Ziess microscope.
Both NotchIC-GFP and GFP transfected cells demonstrated healing of the scratch area; however NotchIC-GFP cells were delayed compared to control. The mean healing rate was 0.96 % +/- 1.3% per hour in the NotchIC-GFP cells compared to 3.42 % +/- 2.4% per hour in GFP cells (P:0.003). The percentage of scratch area covered at 16 hours was 43.06% in GFP transfected cells compared to 14.49% in NotchIC-GFP transfected cells.
Exogenous Notch activation appears to decrease the rate of cornea epithelial cell migration in a scratch assay.
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