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Ashley M. Crane, Andrew Coggin, Hong-Uyen Hua, Katyayini Aribindi, Byron L. Lam, Sanjoy K. Bhattacharya; Mass Spectrometric Lipidomic Analyses of Alkali Exposed Corneal Tissue. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3548.
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To determine whether exposure to sodium hydroxide results in predictable changes in phospholipids in corneal tissue. To determine whether phospholipid profile changes correlate to exposure duration.
Enucleated bovine (n= 40) and cadaver human eyes (n= 6) were procured from Just Meats, Chillicothe, Ohio and Florida Lion Eye Bank, Miami, Florida respectively and exposed to 0 (control), 0.2, 0.5 and 11M sodium hydroxide. The corneas were excised and lipids were extracted using the Bligh and Dyer method, and re-suspended in Isoproyl, Acetonitrile solution. Shotgun lipidomic was performed with a TSQ Quantum Access Max instrument. Briefly, positive mode precursor ion scan (PIS) for phosphocholines (PC; product m/z of 184) and negative mode PIS for phosphoinositol (PI; product m/z 241), phosphoethanolamine (PE; product m/z of 196) and neutral loss scan (NLS) for phosphoserine (PS; NL of 87.1) was used. The samples were infused and scanned for one minute between 200 m/z to 1000 m/z. Ratiometric quantification was performed using quantitative standards for each lipid class. This research adhered to ARVO statement for use of animals in research and Helsinki Declaration.
We found degradation of phospholipids of all classes: Phosphocholines, Phosphoethanolamines, Phosphoserines and Phosphoinositols. Furthermore, duration of exposure provided different amounts of lysophospholipids likely to be the product of phospholipids.
Alkali exposure duration does indeed modify the corneal lipid profile.
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