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Petros C. Karakousis, Thomas A. Albini, Seema M. Thayil, Hossein Nazari Khanamiri, Andrew A. Moshfeghi, Jean-Marie A. Parel, Narsing A. Rao; Hypoxia And Increased VEGF Expression In Experimental Ocular Tuberculosis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3650.
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To study ocular TB pathogenesis in a highly relevant animal model.
Hartley strain guinea pigs were infected via low-dose aerosol with Mycobacterium tuberculosis CDC1551. Ocular pathology was documented by histological exam, mycobacterial culture, acid-fast staining, quantitative PCR for M. tuberculosis DNA, and fundus photography. Immunohistochemistry was performed using monoclonal antibodies against the hypoxia-specific probe pimonidazole and vascular endothelial growth factor (VEGF).
Ocular TB primarily involving the uvea developed in all animals. By Day 56 after infection, fundoscopic examination of animals infected with low-dose aerosol revealed altered vascularization and chorioretinal hemorrhage, and viable bacilli were cultivated from all eyes, despite negative acid-fast staining and M. tuberculosis PCR. Choroidal tuberculous granulomas showed reduced oxygen tension and VEGF expression was detected in the retinal pigment epithelium and photoreceptors.
This model may be useful in elucidating the pathogenesis of ocular TB, as well as in developing tools for diagnosis and assessment of anti-TB treatment responses in the eye.
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