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Haruna Yoshikawa, Morio Ueno, Yoko Ikeda, Tomoko Oya, Kazuhiko Mori, Shigeru Kinoshita; Lipid Peroxidation In Aqueous Humor Of Primary Open Angle Glaucoma And Pseudoexfoliative Glaucoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3842.
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© ARVO (1962-2015); The Authors (2016-present)
4-hydroxynonenal (4-HNE) is one of the aldehydic secondary products of lipid peroxidation, which are generally accepted markers of oxidative stress. Moreover, it has been shown to be capable of binding to proteins and forming stable adducts. Oxidative modification of lipids can be induced during aging and in certain disease conditions. It was reported that primary open angle glaucoma (POAG) and pseudoexfoliative glaucoma (PEG) were caused by oxidative damage to trabecular meshwork cells. The purpose of this present study was to examine oxidative stress in the anterior chamber as a measure of concentrations of 4-HNE in aqueous humor (AH) specimens obtained from glaucoma patients.
AH specimens were directly obtained at the beginning of surgery from 8 patients being treated for POAG and from 4 patients being treated for PEG without any contamination of blood. As control samples, specimens were also collected from 8 glaucoma-free patients at the beginning of cataract surgery. Specimens were separated in relation to patient age, and oxidative stress in the AH specimens was then evaluated by quantifying the 4-HNE-His protein adducts by enzyme immunoassay.
Mean 4-HNE concentrations in AH specimens obtained from the POAG patients were 1.347μg/ml in patients 60-70 years of age, 0.829μg/ml in patients 70-80 years of age, and 0.824μg/ml in patients 80-90 years of age. Those in specimens obtained from the PEG patients were 0.891μg/ml in patients 60-70 years of age, 1.347μg/ml in patients 70-80 years of age, and 0.644μg/ml in patients 80-90 years of age. Those in specimens obtained from the control patients were 0.918μg/ml in patients 60-70 years of age and 1.146μg/ml in patients 70-80 years of age. No significant difference of 4-HNE concentrations was found between the POAG patients, PEG patients, and controls.
The findings of this study show that there was no significant difference of lipid peroxidation in the anterior chamber among POAG, PEG and control subjects in any age distributions.
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