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Coralia C. Luna, Chang He, Jing Wu, Guorong Li, Pratap Challa, Xialin Liu, David L. Epstein, Pedro Gonzalez; Global biochemical Profile of Aqueous Humor from POAG patients and matched controls. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3863.
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To evaluate potential differences between the biochemical profile of aqueous humor (AH) from Primary Open Angle Glaucoma (POAG) patients and age matched controls.
Aqueous humor samples were obtained from patients with a history of POAG and age matched controls at Zhongshan Ophthalmic Centre, Sun Yat-Sen University, Guangzhou, China, following the tenants of the declaration of Helsinki. Samples were grouped by patient condition: control (9) or POAG (9) and processed by Metabolon Inc. Samples were split into equal parts for analysis on the GC/MS and LC/MS/MS platforms. Instrument variability was determined by calculating the median relative standard deviation (RSD) from the internal standards added to each sample. Overall variability was determined by calculating the median RSD for all endogenous metabolites (i.e., non- standards) present in the matrix samples (pooled samples). Welch’s two-sample t-tests were used to evaluate significance (p≤0.05) and the q-value was used to calculate the false discovery rate.
Comparison of global biochemical profiles for aqueous humor from glaucoma and controls revealed statistically significant differences in 31 biochemical compounds, 26 increased and 5 decreased in POAG patients compared to controls. Among the increased metabolites in POAG were, free amino acids (AA) (tryptophan, tyrosine, histidine, methionine, lysine metabolites) and N-acethyl AA (N6-acetyllysine, N-acetylvaline), protein glycation and glycosilation (sialic acid, 2-aminobutyrate), and some drugs and chemical metabolites (1,2 propanediol, dyethanolamine).
Metabolic profile of AH humor from POAG patients suggest several mechanisms by which the observed changes could contribute to the pathophysiology of POAG. Specifically, the observed increase in free AA may reflect reduced uptake or elevated release to aqueous humor under conditions of increased protein breakdown and/or increased solute concentration in the aqueous humor could alter osmotic balance/pressure in the fluid and surrounding tissues. In addition, global metabolic profile might be a promising avenue for the identification of new markers for glaucoma.
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