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Min Hyung Kang, Dong-Jin Oh, Ayan Chatterjee, Douglas J. Rhee; Testican-1 (SPOCK-1) is differentially expressed in Human Ciliary Body and Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3868.
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The matricellular protein Testican-1 has an inhibitory effect on matrix metalloproteinases in non-ocular tissue. It is unknown if Testican-1 is present in the outflow pathways. We hypothesized that Testican-1 would be found in the relevant drainage tissues.
Cultured cells obtained from human ciliary body (CB) and trabecular meshwork (TM) were used. Total RNA was obtained from 4th or 5th passage cultured CB and TM cells, and qRT-PCR was performed using primers specific for Testican-1. Immunoblot analysis was performed to determine the expression of Testican-1 in cultured CB and TM cells.
In qRT-PCR experiments, we found that Testican-1 mRNA was expressed in CB (0.010 ± 0.001 fold, n=4) and TM (0.037 ± 0.010 fold, n=4), respectively, normalized with β-Actin. This indicates that Testican-1 in TM is expressed 4 times higher (p=0.027) than in CB. Immunoblot analysis, demonstrated equivalent expression of Testican-1 in TM and CB (0.48 ± 0.45 fold, n=3).
The findings indicated that Testican-1 mRNA and protein are expressed in cultured CB and TM cells as well as in the anterior segments of normal and glaucomatous eyes. Although the function of Testican-1 is unknown, its function may be related to protease inhibition.
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