Purchase this article with an account.
Kanji Hori, Akira Matsuda, Nobuyuki Ebihara, Toshinari Funaki, Susumu Nakae, Akira Murakami; The Role Of Interleukin-33 (IL-33) In Mouse Experimental Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4000.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
We reported the roles of IL-33, a Th2 type-inflammation initiating cytokine, in human chronic allergic conjunctivitis (IOVS. 50:4646-52). In this study we further analyzed the pathophysiological roles of IL-33, by ragweed-induced experimental allergic (RW) conjunctivitis models and by papain-induced conjunctivitis using IL-33 knockout (KO) mice. (PNAS. 107:18581-18586)
RW-conjunctivitis models were made by alum-RW immunization and three times RW eye drops challenge using IL-33 KO mice (BALB/C background). 24 hours after final challenge, histological analysis was carried out to examine late phase reactions. To examine chronic phase effects of IL-33 for Th2 biased inflammation in the eye, papain-soaked soft contact lens (2mm diameter) were applied to IL-33 KO mice (C57/BL6 background) and the eye lids were sutured. 10 days after contact lens installation, histological analysis was carried out.
RW conjunctivitis model showed that significantly reduced eosinophil infiltration in the IL-33 KO mice’s conjunctiva compared to wild-type control mice. (10.4 ± 5.2 versus 24.0 ± 4.1, mean number of eosinophil ± SD per slides) Papain conjunctivitis model also showed reduced eosinophil infiltration in the IL-33 KO mice’s conjunctiva. (59 in KO mice versus 231 in wild-type mice)
IL-33 plays essential roles for late phase allergic reaction and chronic conjunctival inflammation through facilitating eosinophil infiltration.
This PDF is available to Subscribers Only