March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
A Comparison of the Regions of Increased Autofluorescence Obtained with Near-infrared Autofluorescence and Fundus Autofluorescence in Patients with Retinitis Pigmentosa
Author Affiliations & Notes
  • Mirela R. tabacaru
    Ophthalmology, Bernard and Shirlee Brown Glaucoma Laboratory, Columbia University, New York, New York
  • Tobias Duncker
    Ophthalmology, Columbia University, New York, New York
  • Jonathan P. Greenberg
    Ophthalmology, Columbia University, New York, New York
  • Stephen H. Tsang
    Ophthalmology, Bernard and Shirlee Brown Glaucoma Laboratory, Columbia University, New York, New York
  • Ronald E. Carr
    Ophthalmology, NYU School of Medicine, New York, New York
  • Vivienne C. Greenstein
    Ophthalmology, Columbia University, New York, New York
  • Footnotes
    Commercial Relationships  Mirela R. tabacaru, None; Tobias Duncker, None; Jonathan P. Greenberg, None; Stephen H. Tsang, None; Ronald E. Carr, None; Vivienne C. Greenstein, None
  • Footnotes
    Support  R01 EY018213 (SHT)
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4576. doi:
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      Mirela R. tabacaru, Tobias Duncker, Jonathan P. Greenberg, Stephen H. Tsang, Ronald E. Carr, Vivienne C. Greenstein; A Comparison of the Regions of Increased Autofluorescence Obtained with Near-infrared Autofluorescence and Fundus Autofluorescence in Patients with Retinitis Pigmentosa. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4576.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To compare melanin-related near-infrared autofluorescence (NIR-AF)with lipofuscin-related autofluorescence (FAF), and assess their relationship to the underlying retinal structure in patients with retinitis pigmentosa (RP).

 
Methods:
 

Twelve eyes from 9 patients with RP (autosomal recessive=3, autosomal dominant=1, Usher syndrome type I=2, Usher syndrome type II=3), with ages 7 to 54 years and BCVAs 20/20 to 20/40, were analyzed. For each patient, a horizontal line scan through the fovea was obtained with the Spectralis SLO-OCT (Heidelberg Eng.) and was automatically registered with an FAF (488 nm) image in real time. NIR-AF (787 nm) SLO imaging was performed with the Heidelberg Retina Angiograph 2 (Heidelberg Eng.) and the image was aligned with the FAF image using Matlab software. For 5 eyes, the images were manually registered in Photoshop. The boundaries of the hyperautofluorescent rings seen on NIR-AF and FAF images were compared to the location where the inner segment ellipsoid (ISe) band was not visible.

 
Results:
 

All eyes showed a ring of increased autofluorescence in the same region on both FAF and NIR-AF, but the diameter of the ring was more constricted on NIR-AF than FAF.The outer border of the ring was more distinct on NIR-AF than FAF. Outside the ring, an abrupt decline in intensity was evident on NIR-AF. The inner border of the ring was visualized in all eyes on FAF, however it was less visible in 4 eyes on NIR-AF. On SD-OCT, loss of the ISe band corresponded to the inner border of the ring on both FAF and NIR-AF images, or to an area located within the hyperautofluorescent zone of the ring in the 4 eyes where the inner border was not clear on NIR-AF.

 
Conclusions:
 

A distinct hyperautofluoscent ring is seen on both FAF and NIR-AF. Because the diameter of the ring on NIR-AF correlates with the length of the ISe band seen on SD-OCT and displays a sharply defined outer border, NIR-AF may be a useful tool for monitoring disease progression in RP patients. NIR-AF imaging has an additional merit of being more patient-friendly and not requiring pupil mydriasis. The differences between the NIR-AF and FAF images also provide additional information about the pathophysiological mechanisms in RP.  

 
Keywords: retinal degenerations: hereditary • imaging/image analysis: clinical 
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