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Ana Ojeda, Ravi Munjaal, Peter Lwigale; Expression Of Chemokines During Ocular Development And In The Adult Cornea. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4743.
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To describe and compare the expression patterns of chemokines that may contribute to the maintenance of corneal avascularity in the developing and adult avian and mouse cornea. Chemokines, initially described as cytokines that direct leukocytes in the inflammatory response, also play an important role in angiogenesis and developmental processes. Complementary expression patterns of CXCL14, an angiostatic factor, and CXCL12, a potent angiogenic chemokine, have been found during chick and mouse development. However, very little is known about their expression and function during eye development.
In situ hybridization was performed to assess the expression of the CXCL14, CXCL12 and its receptor CXCR4 during chick eye development at embryonic day (E)3, E5, E7 and E12, and in the mouse eye at E14, E16, post-natal day (P)0 and adult.
In chick embryos, CXCL14 was strongly expressed by stromal keratocytes beginning at E7 and was maintained in the stroma through E12. CXCL12 and its receptor CXCR4 were expressed in angioblasts at E3 and in the periocular region at E5, E7 and E12, where angioblasts aggregate to form blood vessels, but not in the cornea. Surprisingly, CXCL14 was not expressed in the developing and adult mouse cornea. Similar to chick, CXCL12 and CXCR4 were expressed in angioblasts and in the periocular region during mouse eye development.
CXCL14 is expressed at a critical time during the formation of the corneal stroma in chick development and persists in this region at later stages, suggesting that this molecule may contribute to the maintenance of corneal avascularity. However, CXCL14 may not play a similar role in mouse cornea development as seen in the avian model, suggesting evolutionary differences in its role in the anterior eye. The expression patterns of CXCL12 and CXCR4 are conserved in chick and mouse during eye development, suggesting a similar role during vasculogenesis in the periocular region.
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