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Katrin Lorenz, Oliver W. Gramlich, Franz H. Grus, Daniela Ehrlich, Christian Humpel, Josef Troger; Ge-25-like Immunoreactivity In The Rat Eye. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4918.
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To investigate the presence and distribution of the chromogranin A-derived neuropeptide GE-25 in the rat eye.
The molecular form of GE-25 detected by the GE-25 antiserum was evaluated in the rat trigeminal ganglion, retina and remaining tissues of the rat eye by means of western blots and the distribution pattern of GE-25-like immunoreactivity was studied in the rat eye and rat trigeminal ganglion by immunofluorescence.
One single band of approximately 70 kDa was stained in the trigeminal ganglion and retina which represents the uncleaved intact chromogranin A indicating that the proteolytic processing of chromogranin A to GE-25 is limited in these tissues. Sparse GE-25-like immunoreactive nerve fibers were visualized in the corneal stroma, at the limbus around blood vessels, in the sphincter and dilator muscle and stroma of the iris, in the stroma of the ciliary body and ciliary processes and in the stroma and around blood vessels in the choroid. Furthermore, immunoreactivity was present in the corneal endothelium and in Müller glia in the retina which is atypical for neuropeptides. In the trigeminal ganglion GE-25-like immunoreactivity was found in small to medium-sized ganglion cells with a diameter of 20 to 30 µm.
GE-25 like immunoreactivity is distinctly distributed throughout the rat eye and the innervation pattern is predominantly characteristic for neuropeptides. The nerve fibers in the rat eye are obviously of sensory origin.
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