March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
In Vivo Imaging Of A New Indocyanine Green Nanoformulation In An Animal Model
Author Affiliations & Notes
  • Johanna Meyer
    Ophthalmology, University Eye Hospital Bonn, Bonn, Germany
  • Cunea Alexander
    Ophthalmology, University Eye Hospital Bonn, Bonn, Germany
  • Pia Welker
    Mivenion GmbH, Berlin, Germany
  • Kai Licha
    Mivenion GmbH, Berlin, Germany
  • Dagmar Sonntag-Bensch
    Ophthalmology, University Eye Hospital Bonn, Bonn, Germany
  • Steffen Schmitz-Valckenberg
    Ophthalmology, University Eye Hospital Bonn, Bonn, Germany
  • Frank G. Holz
    Ophthalmology, University Eye Hospital Bonn, Bonn, Germany
  • Footnotes
    Commercial Relationships  Johanna Meyer, Heidelberg Engineering, Carl-Zeiss Meditec AG (F); Cunea Alexander, Heidelberg Engineering, Carl Zeiss Meditec AG (F); Pia Welker, Mivenion GmbH (E), Patent WO 2011/095311 (P); Kai Licha, Mivenion GmbH (E), Patent WO 2011/095311 (P); Dagmar Sonntag-Bensch, Heidelberg Engineering, Carl Zeiss Meditec AG (F); Steffen Schmitz-Valckenberg, Heidelberg Engineering (R), Heidelberg Engineering, Carl Zeiss Meditec AG, Optos Ltd., Topcon UK (F); Frank G. Holz, Heidelberg Engineering (R), Heidelberg Engineering, Carl Zeiss Meditec AG, Optos Ltd. (F), Heidelberg Engineering, Carl-Zeiss Meditec AG (C)
  • Footnotes
    Support  German Ministry of Education and Research, FKZ 13N10285
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4991. doi:
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      Johanna Meyer, Cunea Alexander, Pia Welker, Kai Licha, Dagmar Sonntag-Bensch, Steffen Schmitz-Valckenberg, Frank G. Holz; In Vivo Imaging Of A New Indocyanine Green Nanoformulation In An Animal Model. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4991.

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Abstract

Purpose: : To investigate a novel optimized formulation of indocyanine green (nanoICG) in an animal model.

Methods: : ICG was encapsulated with the nonionic solubilizer and emulsifying agent Solutol HS15 to create nanoICG in order to improve the chemical stability and fluorescence efficacy. Using confocal scanning laser ophthalmoscopy (cSLO), in vivo reflectance and fluorescence (excitation 790 nm, emission > 820 nm) imaging was performed in Dark Agouti rats that had undergone argon laser photocoagulation induction of choroidal neovascularizations (CNV). Retinal uptake and fluorescence intensity of both conventional ICG and nanoICG were compared following intravenous injection at three different time points: day 7, 14 and 21 following laser treatment.

Results: : In vivo imaging before dye application showed ill-defined retinal lesions on day 7. Immediately following intravenous dye injection, a strong fluorescence was visible in the retinal vasculature and around the focal laser lesions. Fluorescence intensity in the retinal vasculature was higher for nanoICG compared to conventional ICG at 8 minutes after injection (day 7: p=0,06; day 14: p=0,17; day 21: p=0,05). Over time, a continuous decrease of the fluorescent signal was observed for up to 60 minutes. No fluorescent signal of either nanoICG or ICG was detectable one day after application.

Conclusions: : This study demonstrates that encapsulated ICG can be visualized in the retinal vasculature and laser-induced CNV. Its spatio-temporal kinetics can be studied using in vivo cSLO imaging. Following expanded investigations in animal models, nanoICG may be applicable in patients with retinal and choroidal diseases for earlier and more refined diagnosis.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • choroid: neovascularization • age-related macular degeneration 
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