March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
In Vivo Confocal Microscopy Analysis Of Effects Of Topical Preservative-free Timolol 0.1% Gel On Conjunctiva Changes
Author Affiliations & Notes
  • Paolo Frezzotti
    Ophthalmology, University of Siena, Siena, Italy
  • Paolo Fogagnolo
    Fondazione GB Bietti-IRCCS, Canegrate Milan, Italy
  • Michele M. Iester
    DiNOG, University of Genoa, Genova, Italy
  • Gentiana Haka
    Ophthalmology, University of Siena, Siena, Italy
  • ilaria Motolese
    Ophthalmology, University of Siena, Siena, Italy
  • Pietro Mittica
    Ophthalmology, University of Siena, Siena, Italy
  • Simone Alex Bagaglia
    Ophthalmology, University of Siena, Siena, Italy
  • Cristina Menicacci
    Ophthalmology, University of Siena, Siena, Italy
  • Serena Antenore
    Ophthalmology, University of Siena, Siena, Italy
  • Eduardo Motolese
    Ophthalmology, University of Siena, Siena, Italy
  • Footnotes
    Commercial Relationships  Paolo Frezzotti, None; Paolo Fogagnolo, None; Michele M. Iester, None; Gentiana Haka, None; ilaria Motolese, None; Pietro Mittica, None; Simone Alex Bagaglia, None; Cristina Menicacci, None; Serena Antenore, None; Eduardo Motolese, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5060. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Paolo Frezzotti, Paolo Fogagnolo, Michele M. Iester, Gentiana Haka, ilaria Motolese, Pietro Mittica, Simone Alex Bagaglia, Cristina Menicacci, Serena Antenore, Eduardo Motolese; In Vivo Confocal Microscopy Analysis Of Effects Of Topical Preservative-free Timolol 0.1% Gel On Conjunctiva Changes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5060.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To evaluate the effects at 1 year of preservative-free beta-blocker gel and preservative-containing beta-blocker eye drops on tear secretion and the eye surface.

Methods: : Forty patients with primary open-angle glaucoma or ocular hypertension were divided into two groups according to type of topical hypotensive therapy and compared with 20 healthy age-matched controls. Clinical tests (IOP, Schirmer I test, and lacrimal film break-up time BUT) and in vivo conjunctival confocal microscopy (IVCM) were performed in all patients at baseline and after 12 months. IVCM (HRT II Rostock Cornea Module; Heidelberg Engineering GmbH, Heidelberg, Germany) was performed after topical anaeshesia in the four cardinal locations and at the corresponding limbus to analyze conjunctiva cells.

Results: : A significant reduction in clinical scores (Schirmer I and BUT) was found between groups on topical hypotensive therapy and the control group. The clinical scores were significantly lower in the preserved beta-blocker medication group than in the preservative-free beta-blocker group. Intraepithelial goblet cell density was significantly lower in the preserved than in the preservative-free beta-blocker group (p<0.001) and controls (p<0.001). The epithelial layer was significantly more regular in the preserved beta blocker medication group than in the preservative-free beta-blocker group (p<0.001) and the control group (p<0.001).

Conclusions: : Based on our IVCM data, preservative-free beta-blocker gel induces less changes at ocular surface than preserved beta-blockers, a fact that should be considered to obtain less side effects and maximal adherence to treatment in a chronic condition such as glaucoma.

Keywords: conjunctiva • drug toxicity/drug effects • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×