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Amandio A. Rocha-Sousa, Marta Tavares-Silva, Paulo Pereira-Silva, Sara Azevedo-Pinto, Joana Rodrigues-Araújo, Sonia Pinho, Adelino Leite-Moreira; Ghrelin Is Expressed In The Ciliary Body And Presents A Significant And Sustained Hypotensive Effect In Acute Glaucoma Models. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5110.
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To investigate ghrelin’s role in the modulation of IOP in animal models of acute glaucoma and ghrelin’s and GHSR-1 distribution in the rat’s ocular tissue.
Acute glaucoma was induced in male New Zealand white rabbits (2,0-3,0Kg; n=38) and male Wistar rats (200-300g; n= 17) through an intravitreous injection of 20% NaCl. Afterwards ghrelin was subconjunctivaly injected. In the rabbit, des-acyl ghrelin was also tested, as well as ghrelin’s effect in the presence of L-NAME or ketorolac. Immunolocalization of ghrelin and GHSR-1 was performed in male Wistar rats eye's (200-300g).
Ghrelin, but not des-acyl ghrelin, decreased the IOP in rabbit’s glaucomatous eyes (47.9±11.6%; figure 1). This effect was blunted in the presence of L-NAME and ketorolac. Ghrelin also decreased the IOP in rat’s glaucomatous eyes (41.4±12.2%). Ghrelin was detected in the inner part of ciliary processes, co-localized with lectin (figure 2), while GHSR-1 was detected in the ciliary muscle and choroid.
Ghrelin promotes a decrease in the IOP in acute glaucomatous model eyes. This effect is mediated by GHSR-1a stimulation and prostaglandins or NO release. There appears to be a local system of production and action of Ghrelin and its related peptides.
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