March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Evaluation Of The Surgical Behaviour And Toxicity Of Colored Perfluorocarbon Liquids In Pig Eyes
Author Affiliations & Notes
  • Miguel A. Zapata
    Ophthalmology, Hospital Vall Hebron, Barcelona, Spain
  • Fabio Trinidade
    Ophthalmology, Hospital Vall Hebron, Barcelona, Spain
  • Andrea Carvalho
    Ophthalmology, Hospital Vall Hebron, Barcelona, Spain
  • Anna Salas
    Ophthalmology, Hospital Vall Hebron, Barcelona, Spain
  • Laura Distefano
    Ophthalmology, Hospital Vall Hebron, Barcelona, Spain
  • Jose Garcia-Arumi
    Ophthalmology, Hospital Vall d’Hebron, Barcelona, Spain
  • Footnotes
    Commercial Relationships  Miguel A. Zapata, None; Fabio Trinidade, None; Andrea Carvalho, None; Anna Salas, None; Laura Distefano, None; Jose Garcia-Arumi, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5359. doi:
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      Miguel A. Zapata, Fabio Trinidade, Andrea Carvalho, Anna Salas, Laura Distefano, Jose Garcia-Arumi; Evaluation Of The Surgical Behaviour And Toxicity Of Colored Perfluorocarbon Liquids In Pig Eyes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5359.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The perfluorocarbon liquids (PFCLs) are widely used in vitreoretinal surgery due to their physicochemical properties. Although they’re useful complications have been reported mainly related to their intraocular retention, which can be attributed to the transparent nature of thesen compounds. The use of colored PFCLs would help in a more complete and safe removal of these substances.

Methods: : Twenty-eight eyes of 14 hybrid pigs (15Kg) were used in this experiment. Intraoperative behaviour and toxicity of 2 colored PFCLs (blue and yellow) were evaluated by performing 23G pars plana vitrectomy. In the control group transparent perfluoro-n-octane (PFnO) was used. PFCLs were left in the vitreous cavity for one week according to different surgical protocols: a) 1 - 1.5 ml in a balanced salt solution (BSS) filled eye; b) 0.2 ml with air-fluid exchange at the end; c) 0.1 ml subretinaly. Colour fundus photography, fluorescein angiography and electroretinography (ERG) were performed in all eyes before surgery and euthanasia. One week after surgery eyes were enucleated and fixed in 4% formaldehyde solution, for paraffin inclusion. Serial cuts of 3 μm were stained in standard hematoxiline/eosine to evaluate retinal integrity, number of retinal ganglionar cells, including life and dead cells, nerve fiber layer changes, inner retina vascular pattern and correlation of retina and NFL thickness. To evaluate apoptosis deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) was also performed.

Results: : During the exchanges and under BSS, colored PFCLs were superior to the transparent PFnO. On retinal surface visualization the transparent PFCL was better but both colored PFCLs studied didn’t affect significantly its manipulation. Blue colored PFCL was better seen in the subretinal space than the yellow and the transparent PFnO. Colored PFCLs showed no histological changes when compared to the transparent PFnO. There were no remarkable changes in the ERGs of the compared groups. TUNEL assay was negative in all samples.

Conclusions: : Colored PFCLs facilitate the view during air exchanges and generally provide a better visualization and blue colored PFCL was also better visualized in the subretinal space, which can help to minimize its intraocular retention.

Keywords: retinal detachment • vitreoretinal surgery • retina 
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