March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Preclinical Safety Of Intravitreal Docosahexaenoic Acid In A Rabbit Model
Author Affiliations & Notes
  • Rosa Dolz-Marco
    University and Polytechnic Hospital La Fe, Valencia, Spain
  • Roberto Gallego-Pinazo
    University and Polytechnic Hospital La Fe, Valencia, Spain
  • M Dolores Pinazo-Duran
    "Santiago Grisolia" Ophthalmic Research Unit. "Dr Peset" Hospital, Valencia, Spain
  • Sheila Pons-Vazquez
    "Santiago Grisolia" Ophthalmic Research Unit. "Dr Peset" Hospital, Valencia, Spain
  • Manuel Diaz-Llopis
    University and Polytechnic Hospital La Fe, Valencia, Spain
  • Footnotes
    Commercial Relationships  Rosa Dolz-Marco, None; Roberto Gallego-Pinazo, None; M Dolores Pinazo-Duran, None; Sheila Pons-Vazquez, None; Manuel Diaz-Llopis, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5363. doi:
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      Rosa Dolz-Marco, Roberto Gallego-Pinazo, M Dolores Pinazo-Duran, Sheila Pons-Vazquez, Manuel Diaz-Llopis; Preclinical Safety Of Intravitreal Docosahexaenoic Acid In A Rabbit Model. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5363.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the retinal toxicity of intravitreal administration of the purified fatty acid docosahexaenoic (DHA) in rabbit eyes.

Methods: : Sixteen New Zealand albino rabbits were selected. Six concentrations of DHA (Brudy Laboratories, Barcelona, Spain) were prepared: 10, 5 and 2.5 µg/1 µL; and 50, 25, and 5 µg/50 µL. A volume of 0.05 ml of each concentration was injected intravitreally in the right eye of 2 rabbits. As a control, 0.05 ml of saline solution was injected into in the right eye of 4 animals. Retinal safety of intravitreal DHA was assesed by electroretinography (ERG) -changes between before and 1 week after the injection-; and by histologic examination of the retinal samples obtained after sacrificing the rabbits.

Results: : We evidenced a severe intraocular inflammation in eyes treated with 10 µg/1 µL, 5 µg/1 µL and 2’5 µg/1 µL DHA concentrations. The ERG studies -amplitude and time of A and B waves- did not show significant difference (p< 0,01) between control and DHA-injected eyes. The histologic examination did not evidence any retinal abnormality in the rabbits injected with different concentrations of DHA.

Conclusions: : DHA may be a safe intravitreal drug in the rabbit model up to 50 µg/50 µL. Further studies are warranted to evaluate the safety and efficacy in human.

Keywords: drug toxicity/drug effects • retina • antioxidants 
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