March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Evaluating Retinal Toxicity of a New Heavy Intraocular Dye Using the Model of Perfused and Isolated Retinal Cultures of Human Origin
Author Affiliations & Notes
  • Sebastian Mueller
    Department of Ophthalmology I, Tuebingen, Germany
  • Kai Januschowski
    Department of Ophthalmology I, Tuebingen, Germany
  • Martin Spitzer
    Department of Ophthalmology I, Tuebingen, Germany
  • Charlotte Schramm
    Department of Ophthalmology I, Tuebingen, Germany
  • Karl-Ulrich Bartz-Schmidt
    Department of Ophthalmology I, Tuebingen, Germany
  • Peter Szurman
    Department of Ophthalmology I, Tuebingen, Germany
  • Footnotes
    Commercial Relationships  Sebastian Mueller, None; Kai Januschowski, None; Martin Spitzer, None; Charlotte Schramm, None; Karl-Ulrich Bartz-Schmidt, None; Peter Szurman, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5373. doi:
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      Sebastian Mueller, Kai Januschowski, Martin Spitzer, Charlotte Schramm, Karl-Ulrich Bartz-Schmidt, Peter Szurman; Evaluating Retinal Toxicity of a New Heavy Intraocular Dye Using the Model of Perfused and Isolated Retinal Cultures of Human Origin. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5373.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

During vitreoretinal surgery vital dyes such as Brilliant Blue (BBG) are used to visualize anatomical structures. By adding deuteriumoxide (D2O) many surgeons try to create a dye mixture heavier than water to facilitate staining of the inner limiting membrane (ILM) without preceding fluid-air exchange. However, the intraocular use of D2O is critical. This study investigated the effect of 0.4 ml BBG (Brilliant Peel™ 0.25 mg/ml, Fluoron, Ulm, Germany) mixed with 0.13ml/ml D2O on retinal function of a pseudo in vivo model using human whole mount cultures.

 
Methods:
 

Human retinas were isolated and superfused with an oxygen saturated nutrient solution before the electroretinogram (ERG) was recorded. BBG mixed with 0.13 ml/ml D2O was applied epiretinally for a staining period of 60 seconds. ERG-recovery was monitored for 75 minutes. For obtaining a-waves1 mM aspartate was added to the nutrient solution.

 
Results:
 

Reductions of the a- and b-wave amplitude were found directly after exposure with BBG in all test series. These effects on the electroretinogram were rapidly and completely reversible within the recovery time for all exposure times. Between the ERG-amplitudes no differences were found before and after dye application at the end of the washout.

 
Conclusions:
 

The clinically used mixture of BBG and 0.13 ml/ml D2O seems to be safe for clinical use. Longer staining periods than 60 seconds were not tested.  

 
Keywords: drug toxicity/drug effects • retinal glia • retina 
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