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Yoshihiko Usui, Yoshihiro Wakabayashi, Yoko Okunuki, Hideki Mori, Takeshi Kezuka, Hiroshi Goto; Immune Mediators In Vitreous Fluids From Patients With Vitreoretinal B Cell Lymphoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5471.
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© ARVO (1962-2015); The Authors (2016-present)
Various immune mediators are hypothesized to play important roles in the pathogenesis of vitreoretinal B cell lymphoma, although the exact mechanisms remain unclear. We determined the immune mediator profile in the vitreous of eyes with vitreoretinal B cell lymphoma.
Twenty-eight eyes (23 patients) with vitreoretinal B cell lymphoma were studied. Twenty-seven eyes (27 patients) undergoing vitrectomy for macular hole and epiretinal membrane served as controls. Undiluted vitreous samples were collected, and cytometric bead array and ELISA were used to determine the concentrations of 38 immune mediators including 14 interleukins (IL), interferon (IFN)-γ, oncostatin M (OSM), interferon-γ-inducible protein (IP)-10, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1α, MIP-1β, regulated on activation, normal T cell expressed and secreted (RANTES), monokine induced by interferon-γ (Mig), stromal cell-derived factor (SDF)-1α, B cell-attracting chemokine (BCA)-1, basic fibroblast growth factor (bFGF), Fas ligand, granzyme A, and granzyme B.
Vitreous levels of BCA-1, bFGF, Fas ligand, granzyme A, granzyme B, IFN-γ, IL-6, IL-8, IL-10, IP-10, MCP-1, Mig, MIP-1α, MIP-1β, OSM, RANTES, and SDF-1α were significantly higher in vitreoretinal B cell lymphoma patients than in controls. A moderate to strong positive correlation was observed between granzyme A and BCA-1, IFN-γ, or MIP-1β; IFN-γ and Mig or SDF-1α; IL-6 and IL-8, IL-10, IP-10, or MCP-1; IL-8 and MCP-1, Mig, or MIP-1β; IL-10 and MCP-1 or MIP-1α; Mig and IP-10 or Mig; and MIP-1α and MIP-1β.
The present study suggests that elevated vitreous levels of various immune mediators inducing growth, migration, and apoptosis of B cell lymphoma are possibly involved in the pathophysiology of vitreoretinal B cell lymphoma.
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